CASP9 sirna

CASP9 siRNA (Mouse)

Cat. Num. AAA8225427

• Gene Names: Casp9; Mch6; APAF-3; CASP-9; AI115399; AW493809; ICE-LAP6; Caspase-9
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CASP9; CASP9 siRNA (Mouse); MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6; CASP9 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CASP9 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 454

• Applicable Applications for CASP9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CASP9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CASP9 sirna

siRNA to inhibit CASP9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31560479
• NCBI GeneID: 12371
• NCBI Accession #: NP_056548.2
• NCBI GenBank Nucleotide #: NM_015733.5
• UniProt Accession #: Q8C3Q9
• Molecular Weight: 49,979 Da
• NCBI Official Full Name: caspase-9 isoform 1
• NCBI Official Synonym Full Names: caspase 9
• NCBI Official Symbol: Casp9
• NCBI Official Synonym Symbols: Mch6; APAF-3; CASP-9; AI115399; AW493809; ICE-LAP6; Caspase-9
• NCBI Protein Information: caspase-9
• UniProt Protein Name: Caspase-9
• Protein Family: Caspase
• UniProt Gene Name: Casp9
• UniProt Synonym Gene Names: Mch6; CASP-9; APAF-3; ICE-LAP6
• UniProt Entry Name: CASP9_MOUSE

NCBI Description

This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, the initiator caspase, is activated after cytochrome c release from mitochondria and targets downstream effectors. In mouse, deficiency of this gene can cause perinatal lethality. This protein may have a role in normal brain development. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013]

Uniprot Description

CASP9: a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two isoforms.

Protein type: Protease; Apoptosis; EC 3.4.22.62

Cellular Component: mitochondrion; cytoplasm; intracellular; nucleus; cytosol; apoptosome

Molecular Function: peptidase activity; protein binding; hydrolase activity; cysteine-type endopeptidase activity; protein kinase binding; SH3 domain binding; cysteine-type peptidase activity

Biological Process: caspase activation; regulation of apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of apoptosis; apoptosis; positive regulation of neuron apoptosis; DNA damage response, signal transduction; proteolysis; response to DNA damage stimulus; response to organic cyclic substance; response to UV