WASH1 sirna

WASH1 siRNA (Mouse)

Cat. Num. AAA8225346

• Gene Names: Wash1; Wash; ORF19; 1110049F14Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WASH1; WASH1 siRNA (Mouse); ORF19; WASH; WAS protein family homolog 1; WASH1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: WASH1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 475

• Applicable Applications for WASH1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WASH1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WASH1 sirna

siRNA to inhibit WASH1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 83649762
• NCBI GeneID: 68767
• NCBI Accession #: NP_001032846.1
• NCBI GenBank Nucleotide #: NM_001037757.1
• UniProt Accession #: Q8VDD8; Q3U473
• Molecular Weight: 51,659 Da
• NCBI Official Full Name: WAS protein family homolog 1
• NCBI Official Synonym Full Names: WAS protein family homolog 1
• NCBI Official Symbol: Wash1
• NCBI Official Synonym Symbols: Wash; ORF19; 1110049F14Rik
• NCBI Protein Information: WAS protein family homolog 1
• UniProt Protein Name: WAS protein family homolog 1
• Protein Family: WAS protein family
• UniProt Gene Name: Wash1
• UniProt Synonym Gene Names: Orf19; Wash
• UniProt Entry Name: WASH1_MOUSE

Uniprot Description

WASH1: Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Belongs to the WASH1 family.

Cellular Component: centrosome; recycling endosome; cytoskeleton; membrane; cell; cytoplasm; early endosome; exocyst; autophagic vacuole; cytoplasmic vesicle; intracellular; endosome

Molecular Function: gamma-tubulin binding; ubiquitin protein ligase binding; actin binding; alpha-tubulin binding

Biological Process: negative regulation of autophagy; regulation of immune response; exocytosis; regulation of protein ubiquitination; endosome transport; oocyte maturation; dendritic cell antigen processing and presentation; T cell proliferation; extracellular matrix disassembly; negative regulation of phosphoinositide 3-kinase activity; positive regulation of pseudopodium formation; transport; polar body extrusion after meiotic divisions; retrograde transport, endosome to Golgi; positive regulation of cell migration