CTSZ sirna

CTSZ siRNA (Human)

Cat. Num. AAA8225230

• Gene Names: CTSZ; CTSX
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CTSZ; CTSZ siRNA (Human); Cathepsin Z; Cathepsin P; Cathepsin X; CTSZ sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CTSZ siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 303

• Applicable Applications for CTSZ sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CTSZ gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CTSZ sirna

siRNA to inhibit CTSZ expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22538442
• NCBI GeneID: 1522
• NCBI Accession #: NP_001327.2
• NCBI GenBank Nucleotide #: NM_001336.3
• UniProt Accession #: Q9UBR2; O75331; Q9UQV5; Q9UQV6; B2RC40
• Molecular Weight: 33,868 Da
• NCBI Official Full Name: cathepsin Z preproprotein
• NCBI Official Synonym Full Names: cathepsin Z
• NCBI Official Symbol: CTSZ
• NCBI Official Synonym Symbols: CTSX
• NCBI Protein Information: cathepsin Z
• UniProt Protein Name: Cathepsin Z
• Protein Family: Cathepsin
• UniProt Gene Name: CTSZ
• UniProt Entry Name: CATZ_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase and member of the peptidase C1 family. It exhibits both carboxy-monopeptidase and carboxy-dipeptidase activities. The encoded protein has also been known as cathepsin X and cathepsin P. This gene is expressed ubiquitously in cancer cell lines and primary tumors and, like other members of this family, may be involved in tumorigenesis. [provided by RefSeq, Oct 2008]

Uniprot Description

CTSZ: Exhibits carboxy-monopeptidase as well as carboxy- dipeptidase activity. Belongs to the peptidase C1 family.

Protein type: Protease; EC 3.4.18.1

Chromosomal Location of Human Ortholog: 20q13.32

Cellular Component: extracellular space; intracellular membrane-bound organelle; lysosome; endoplasmic reticulum; plasma membrane

Molecular Function: cysteine-type peptidase activity

Biological Process: cellular protein metabolic process; proteolysis; angiotensin maturation

Disease: Mycobacterium Tuberculosis, Susceptibility To