BMPER sirna

BMPER siRNA (Human)

Cat. Num. AAA8224523

• Gene Names: BMPER; CV2; CV-2; CRIM3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BMPER; BMPER siRNA (Human); KIAA1965; BMP-binding endothelial regulator protein; Bone morphogenetic protein-binding endothelial cell precursor-derived regulator; Protein crossveinless-2; hCV2; BMPER sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: BMPER siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 685

• Applicable Applications for BMPER sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BMPER gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BMPER sirna

siRNA to inhibit BMPER expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 37693519
• NCBI GeneID: 168667
• NCBI Accession #: NP_597725.1
• NCBI GenBank Nucleotide #: NM_133468.4
• UniProt Accession #: Q8N8U9; Q8TF36; A8K1P8
• Molecular Weight: 75,997 Da
• NCBI Official Full Name: BMP-binding endothelial regulator protein
• NCBI Official Synonym Full Names: BMP binding endothelial regulator
• NCBI Official Symbol: BMPER
• NCBI Official Synonym Symbols: CV2; CV-2; CRIM3
• NCBI Protein Information: BMP-binding endothelial regulator protein
• UniProt Protein Name: BMP-binding endothelial regulator protein
• Protein Family: BMP-binding endothelial regulator protein
• UniProt Gene Name: BMPER
• UniProt Synonym Gene Names: KIAA1965; hCV2
• UniProt Entry Name: BMPER_HUMAN

NCBI Description

This gene encodes a secreted protein that interacts with, and inhibits bone morphogenetic protein (BMP) function. It has been shown to inhibit BMP2- and BMP4-dependent osteoblast differentiation and BMP-dependent differentiation of the chondrogenic cells. Mutations in this gene are associated with a lethal skeletal disorder, diaphanospondylodysostosis. [provided by RefSeq, Dec 2011]

Uniprot Description

BMPER: Inhibitor of bone morphogenetic protein (BMP) function, it may regulate BMP responsiveness of osteoblasts and chondrocytes. Defects in BMPER are the cause of diaphanospondylodysostosis (DSD). A rare, recessively inherited, perinatal lethal skeletal disorder. The primary skeletal characteristics of the phenotype include a small chest, abnormal vertebral segmentation, and posterior rib gaps containing incompletely differentiated mesenchymal tissue. Consistent craniofacial features include ocular hypertelorism, epicanthal folds, a depressed nasal bridge with a short nose, and low-set ears. The most commonly described extraskeletal finding is nephroblastomatosis with cystic kidneys, but other visceral findings have been described in some cases.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: extracellular space

Biological Process: endothelial cell differentiation; blood vessel endothelial cell proliferation during sprouting angiogenesis; ureteric bud development; inner ear development; endothelial cell activation; negative regulation of BMP signaling pathway

Disease: Diaphanospondylodysostosis