RUNX1T1 sirna

RUNX1T1 siRNA (Human)

Cat. Num. AAA8224366

• Gene Names: RUNX1T1; CDR; ETO; MTG8; AML1T1; ZMYND2; CBFA2T1; AML1-MTG8
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RUNX1T1; RUNX1T1 siRNA (Human); AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2; Protein CBFA2T1; Cyclin-D-related protein; Eight twenty one protein; Protein ETO; Protein MTG8; Zinc finger MYND domain-containing protein 2; RUNX1T1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RUNX1T1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 577

• Applicable Applications for RUNX1T1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RUNX1T1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RUNX1T1 sirna

siRNA to inhibit RUNX1T1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 310923090
• NCBI GeneID: 862
• NCBI Accession #: NP_001185554.1
• NCBI GenBank Nucleotide #: NM_001198625.1
• UniProt Accession #: Q06455; O14784; Q06456; Q14873; Q16239; Q16346; Q16347; Q6IBL1; Q6NXH1; Q7Z4J5; B7Z4P4; E7EPN4
• Molecular Weight: 63,212 Da
• NCBI Official Full Name: protein CBFA2T1 isoform A
• NCBI Official Synonym Full Names: runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
• NCBI Official Symbol: RUNX1T1
• NCBI Official Synonym Symbols: CDR; ETO; MTG8; AML1T1; ZMYND2; CBFA2T1; AML1-MTG8
• NCBI Protein Information: protein CBFA2T1
• UniProt Protein Name: Protein CBFA2T1
• Protein Family: Protein
• UniProt Gene Name: RUNX1T1
• UniProt Synonym Gene Names: AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2
• UniProt Entry Name: MTG8_HUMAN

NCBI Description

This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]

Uniprot Description

RUNX1T1: Transcription regulator that excerts its function by binding to histone deacetylases and transcription factors. Can repress transactivation mediated by TCF12. Homotetramer. Heterotetramer with CBFA2T2 and CBFA2T3. Interacts with TCF12, SIN3A, HDAC1, HDAC2, HDAC3, NCOR1 and NCOR2. Interacts with ATN1 (via its N-terminus); the interaction enhances the transcriptional repression. Most abundantly expressed in brain. Lower levels in lung, heart, testis and ovary. Belongs to the CBFA2T family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation; Oncoprotein

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: nucleoplasm; nuclear matrix; mitochondrion; cytoplasm

Molecular Function: identical protein binding; protein binding; DNA binding; metal ion binding; transcription corepressor activity; transcription factor activity

Biological Process: generation of precursor metabolites and energy; transcription, DNA-dependent; negative regulation of transcription, DNA-dependent