CDKN1B sirna

CDKN1B siRNA (Mouse)

Cat. Num. AAA8224355

• Gene Names: Cdkn1b; p27; Kip1; p27Kip1; AA408329; AI843786
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDKN1B; CDKN1B siRNA (Mouse); Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1; CDKN1B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CDKN1B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 197

• Applicable Applications for CDKN1B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CDKN1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDKN1B sirna

siRNA to inhibit CDKN1B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31542372
• NCBI GeneID: 12576
• NCBI Accession #: NP_034005.2
• NCBI GenBank Nucleotide #: NM_009875.4
• UniProt Accession #: P46414; Q8BG74
• Molecular Weight: 22,193 Da
• NCBI Official Full Name: cyclin-dependent kinase inhibitor 1B
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 1B
• NCBI Official Symbol: Cdkn1b
• NCBI Official Synonym Symbols: p27; Kip1; p27Kip1; AA408329; AI843786
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1B
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1B
• Protein Family: Cyclin-dependent kinase inhibitor
• UniProt Gene Name: Cdkn1b
• UniProt Entry Name: CDN1B_MOUSE

Uniprot Description

p27Kip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. May mediate TGF beta-induced g1 arrest. Its degradation is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Protein type: Inhibitor; Cell cycle regulation; Oncoprotein; Protein kinase, regulatory subunit

Cellular Component: protein complex; cytoplasm; cytosol; nucleus; endosome

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; protein kinase inhibitor activity; chaperone binding; protein complex binding; caspase activator activity; Hsp70 protein binding; protein phosphatase binding

Biological Process: caspase activation; cell death; negative regulation of kinase activity; positive regulation of microtubule polymerization; negative regulation of cyclin-dependent protein kinase activity; negative regulation of cell motility; cell cycle; regulation of cell proliferation; negative regulation of cell proliferation; sensory perception of sound; positive regulation of cell proliferation; autophagic cell death; negative regulation of phosphorylation; negative regulation of mitotic cell cycle; negative regulation of cell growth; cell cycle arrest; negative regulation of transcription, DNA-dependent; inner ear development; potassium ion transport; negative regulation of epithelial cell proliferation; negative regulation of apoptosis; G1/S transition of mitotic cell cycle