Western Blot (WB)
(Mouse Cdkn1b Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Mouse Cdkn1b antibody detected the Mouse Cdkn1b protein (arrow).)
Rabbit anti-Human, Mouse Cdkn1b Polyclonal Antibody | anti-Cdkn1b antibody
Cdkn1b, NT (Cdkn1b, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1) (AP)
Gene Names
Cdkn1b; p27; Kip1; p27Kip1; AA408329; AI843786
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Cdkn1b; Polyclonal Antibody; NT (Cdkn1b; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1) (AP); p27Kip1; anti-Cdkn1b antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-Cdkn1b antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Mouse Cdkn1b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of mouse Cdkn1b.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Mouse Cdkn1b Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Mouse Cdkn1b antibody detected the Mouse Cdkn1b protein (arrow).)
Western Blot (WB)
(Mouse Cdkn1b Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Mouse Cdkn1b antibody detected the Mouse Cdkn1b protein (arrow).)
Related Product Information for anti-Cdkn1b antibody
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E-and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.
Product Categories/Family for anti-Cdkn1b antibody
References
Singh, A., et al. Mol. Cell. Biol. 30(21):5145-5159(2010). Antico-Arciuch, V.G., et al. Oncogene 29(42):5678-5686(2010). Mitsuhashi, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(37):16331-16335(2010). Zhang, Y., et al. Genes Dev. 24(16):1746-1757(2010). Wang, Y.Y., et al. J Mol Cell Biol 2(4):209-216(2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,193 Da
NCBI Official Full Name
cyclin-dependent kinase inhibitor 1B
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 1B
NCBI Official Symbol
Cdkn1b
NCBI Official Synonym Symbols
p27; Kip1; p27Kip1; AA408329; AI843786
NCBI Protein Information
cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor p27
UniProt Protein Name
Cyclin-dependent kinase inhibitor 1B
Protein Family
UniProt Gene Name
Cdkn1b
UniProt Entry Name
CDN1B_MOUSE
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Product Notes
The Cdkn1b cdkn1b (Catalog #AAA6284633) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cdkn1b, NT (Cdkn1b, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cdkn1b can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Cdkn1b cdkn1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cdkn1b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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