FEN1 sirna

FEN1 siRNA (Rat)

Cat. Num. AAA8223612

• Gene Names: Fen1; FEN-1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FEN1; FEN1 siRNA (Rat); Flap endonuclease 1; FEN-1; Flap structure-specific endonuclease 1; FEN1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: FEN1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 380

• Applicable Applications for FEN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat FEN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FEN1 sirna

siRNA to inhibit FEN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758170
• NCBI GeneID: 84490
• NCBI Accession #: NP_445882.1
• NCBI GenBank Nucleotide #: NM_053430.1
• UniProt Accession #: Q5XIP6; Q9JHW7
• Molecular Weight: 42,609 Da
• NCBI Official Full Name: flap endonuclease 1
• NCBI Official Synonym Full Names: flap structure-specific endonuclease 1
• NCBI Official Symbol: Fen1
• NCBI Official Synonym Symbols: FEN-1
• NCBI Protein Information: flap endonuclease 1
• UniProt Protein Name: Flap endonuclease 1
• UniProt Gene Name: Fen1
• UniProt Synonym Gene Names: FEN-1
• UniProt Entry Name: FEN1_RAT

NCBI Description

removes RNA primers during Okazaki fragment maturation in DNA synthesis [RGD, Feb 2006]

Uniprot Description

FEN1: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300. Interacts with DDX11. Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Protein type: Mitochondrial; DNA repair, damage; Ribonuclease; Hydrolase; Enzyme, misc.; DNA-binding; DNA replication; Deoxyribonuclease; Nuclear receptor co-regulator; EC 3.1.-.-; Nucleolus

Cellular Component: nucleoplasm; mitochondrion; membrane; nucleolus; plasma membrane; nucleus

Molecular Function: 5'-3' exonuclease activity; DNA binding; 5'-flap endonuclease activity; manganese ion binding; exonuclease activity; magnesium ion binding; ribonuclease H activity; flap endonuclease activity

Biological Process: DNA replication, removal of RNA primer; base-excision repair; DNA repair; DNA replication; DNA catabolic process, endonucleolytic; memory