ATP7B sirna

ATP7B siRNA (Human)

Cat. Num. AAA8223581

• Gene Names: ATP7B; WD; PWD; WC1; WND
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATP7B; ATP7B siRNA (Human); PWD; WC1; WND; Copper-transporting ATPase 2; Copper pump 2; Wilson disease-associated protein; ATP7B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ATP7B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1465

• Applicable Applications for ATP7B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ATP7B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATP7B sirna

siRNA to inhibit ATP7B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 55743071
• NCBI GeneID: 540
• NCBI Accession #: NP_000044.2
• NCBI GenBank Nucleotide #: NM_000053.3
• UniProt Accession #: P35670; Q16318; Q16319; Q4U3V3; Q59FJ9; Q5T7X7
• Molecular Weight: 155,125 Da
• NCBI Official Full Name: copper-transporting ATPase 2 isoform a
• NCBI Official Synonym Full Names: ATPase, Cu++ transporting, beta polypeptide
• NCBI Official Symbol: ATP7B
• NCBI Official Synonym Symbols: WD; PWD; WC1; WND
• NCBI Protein Information: copper-transporting ATPase 2
• UniProt Protein Name: Copper-transporting ATPase 2
• UniProt Gene Name: ATP7B
• UniProt Synonym Gene Names: PWD; WC1; WND
• UniProt Entry Name: ATP7B_HUMAN

NCBI Description

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq, Jul 2008]

Uniprot Description

ATP7B: Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. Monomer. Interacts with COMMD1/MURR1. Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.6.3.54; Hydrolase; Transporter, ion channel; Membrane protein, multi-pass; Transporter; Vesicle

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: Golgi membrane; mitochondrion; membrane; perinuclear region of cytoplasm; integral to plasma membrane; basolateral plasma membrane; cytoplasmic membrane-bound vesicle; late endosome; trans-Golgi network

Molecular Function: protein binding; copper ion binding; copper-exporting ATPase activity; ATP binding

Biological Process: lactation; cellular copper ion homeostasis; response to copper ion; metabolic process; sequestering of calcium ion; cellular zinc ion homeostasis; copper ion import; copper ion transport; copper ion export; transmembrane transport; intracellular copper ion transport

Disease: Wilson Disease