ARHGEF15 sirna

ARHGEF15 siRNA (Human)

Cat. Num. AAA8222305

• Gene Names: ARHGEF15; E5; ARGEF15; Ephexin5; Vsm-RhoGEF
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ARHGEF15; ARHGEF15 siRNA (Human); KIAA0915; Rho guanine nucleotide exchange factor 15; Ephexin-5; E5; Vsm-RhoGEF; ARHGEF15 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ARHGEF15 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 841

• Applicable Applications for ARHGEF15 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ARHGEF15 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ARHGEF15 sirna

siRNA to inhibit ARHGEF15 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 302191621
• NCBI GeneID: 22899
• NCBI Accession #: NP_079290.1
• NCBI GenBank Nucleotide #: NM_025014.1
• UniProt Accession #: O94989; Q8N449; Q9H8B4; A8K6G1
• NCBI Official Full Name: rho guanine nucleotide exchange factor 15
• NCBI Official Synonym Full Names: Rho guanine nucleotide exchange factor (GEF) 15
• NCBI Official Symbol: ARHGEF15
• NCBI Official Synonym Symbols: E5; ARGEF15; Ephexin5; Vsm-RhoGEF
• NCBI Protein Information: rho guanine nucleotide exchange factor 15
• UniProt Protein Name: Rho guanine nucleotide exchange factor 15
• Protein Family: Rho guanine nucleotide exchange factor
• UniProt Gene Name: ARHGEF15
• UniProt Synonym Gene Names: KIAA0915; E5
• UniProt Entry Name: ARHGF_HUMAN

NCBI Description

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein-coupled receptors. This gene encodes a protein that functions as a specific guanine nucleotide exchange factor for RhoA. It also interacts with ephrin A4 in vascular smooth muscle cells. Two alternatively spliced transcripts variants that encode the same protein have been found for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

ephexin-5: Specific GEF for RhoA activation. Does not activate RAC1 or CDC42. Regulates vascular smooth muscle contractility. Negatively regulates excitatory synapse development by suppressing the synapse-promoting activity of EPHB2.

Protein type: GEFs, Rac/Rho; GEFs

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: dendrite; cytoplasm

Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity; GTPase activator activity

Biological Process: regulation of catalytic activity; positive regulation of stress fiber formation