Western Blot (WB)
(Western blot analysis of TRAF3IP3 expression in MDAMB453 (A), Jurkat (B), HeLa (C) whole cell lysates.)
Rabbit TRAF3IP3 Polyclonal Antibody | anti-TRAF3IP3 antibody
Anti-TRAF3IP3 Antibody
Western Blot (WB)
(Western blot analysis of TRAF3IP3 expression in MDAMB453 (A), Jurkat (B), HeLa (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of TRAF3IP3 expression in MDAMB453 (A), Jurkat (B), HeLa (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of TRAF3IP3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of TRAF3IP3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunoprecipitation (IP)
(Immunoprecipitation of TRAF3IP3 from 0.5mg Mouse cortex tissue lysate, using 5ug of anti-TRAF3IP3 Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse cortex extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with anti-TRAF3IP3 Antibody.)
Immunoprecipitation (IP)
(Immunoprecipitation of TRAF3IP3 from 0.5mg Mouse cortex tissue lysate, using 5ug of anti-TRAF3IP3 Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse cortex extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with anti-TRAF3IP3 Antibody.)
NCBI and Uniprot Product Information
NCBI Description
The gene encodes a protein that mediates cell growth by modulating the c-Jun N-terminal kinase signal transduction pathway. The encoded protein may also interact with a large multi-protein assembly containing the phosphatase 2A catalytic subunit. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
Uniprot Description
TRAF3IP3: May function as an adapter molecule that regulates TRAF3-mediated JNK activation. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q32
Cellular Component: integral to membrane
Molecular Function: protein binding
Research Articles on TRAF3IP3
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Product Notes
The TRAF3IP3 traf3ip3 (Catalog #AAA822211) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TRAF3IP3 Antibody reacts with Human, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's TRAF3IP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100). Researchers should empirically determine the suitability of the TRAF3IP3 traf3ip3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRAF3IP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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