GJB4 sirna

GJB4 siRNA (Human)

Cat. Num. AAA8221680

• Gene Names: GJB4; EKV; CX30.3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GJB4; GJB4 siRNA (Human); Gap junction beta-4 protein; Connexin-30.3; Cx30.3; GJB4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GJB4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 266

• Applicable Applications for GJB4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GJB4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GJB4 sirna

siRNA to inhibit GJB4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 23397464
• NCBI GeneID: 127534
• NCBI Accession #: NP_694944.1
• NCBI GenBank Nucleotide #: NM_153212.2
• UniProt Accession #: Q9NTQ9; B3KQ82
• Molecular Weight: 30,419 Da
• NCBI Official Full Name: gap junction beta-4 protein
• NCBI Official Synonym Full Names: gap junction protein, beta 4, 30.3kDa
• NCBI Official Symbol: GJB4
• NCBI Official Synonym Symbols: EKV; CX30.3
• NCBI Protein Information: gap junction beta-4 protein
• UniProt Protein Name: Gap junction beta-4 protein
• Protein Family: Gap junction beta-4 protein
• UniProt Gene Name: GJB4
• UniProt Synonym Gene Names: Cx30.3
• UniProt Entry Name: CXB4_HUMAN

NCBI Description

This gene encodes a transmembrane connexin protein that is a component of gap junctions. Mutations in this gene have been associated with erythrokeratodermia variabilis, progressive symmetric erythrokeratoderma and hearing impairment. [provided by RefSeq, Dec 2009]

Uniprot Description

GJB4: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJB4 are a cause of erythrokeratodermia variabilis (EKV). EKV is a genodermatosis characterized by the appearance of two independent skin lesions: transient figurate erythematous patches and hyperkeratosis that is usually localized but occasionally occurs in its generalized form. Clinical presentation varies significantly within a family and from one family to another. Palmoplantar keratoderma is present in around 50% of cases. Belongs to the connexin family. Beta-type (group I) subfamily.

Protein type: Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: connexon complex; integral to membrane

Biological Process: olfactory behavior; sensory perception of smell; cell communication

Disease: Erythrokeratodermia Variabilis Et Progressiva