PDE4DIP sirna

PDE4DIP siRNA (Human)

Cat. Num. AAA8221634

• Gene Names: PDE4DIP; MMGL; CMYA2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PDE4DIP; PDE4DIP siRNA (Human); CMYA2; KIAA0454; KIAA0477; MMGL; Myomegalin; Cardiomyopathy-associated protein 2; Phosphodiesterase 4D-interacting protein; PDE4DIP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PDE4DIP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 173

• Applicable Applications for PDE4DIP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PDE4DIP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDE4DIP sirna

siRNA to inhibit PDE4DIP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 50658077
• NCBI GeneID: 9659
• NCBI Accession #: NP_001002810.1
• NCBI GenBank Nucleotide #: NM_001002810.3
• UniProt Accession #: Q5VU43; O75042; O75065; Q2YDC1; Q5VU42; Q5VU44; Q5VU45; Q5VU46; Q5VU47; Q5VU48; Q5VU49; A2RU15
• Molecular Weight: 28,507 Da
• NCBI Official Full Name: myomegalin isoform 4
• NCBI Official Synonym Full Names: phosphodiesterase 4D interacting protein
• NCBI Official Symbol: PDE4DIP
• NCBI Official Synonym Symbols: MMGL; CMYA2
• NCBI Protein Information: myomegalin
• UniProt Protein Name: Myomegalin
• Protein Family: Myomegalin
• UniProt Gene Name: PDE4DIP
• UniProt Synonym Gene Names: CMYA2; KIAA0454; KIAA0477; MMGL
• UniProt Entry Name: MYOME_HUMAN

NCBI Description

The protein encoded by this gene serves to anchor phosphodiesterase 4D to the Golgi/centrosome region of the cell. Defects in this gene may be a cause of myeloproliferative disorder (MBD) associated with eosinophilia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

PDE4DIP iso1: May function as an anchor sequestering components of the cAMP-dependent pathway to Golgi and/or centrosomes. A chromosomal aberration involving PDE4DIP may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP- PDGFRB fusion protein. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q12

Cellular Component: Golgi apparatus; centrosome; myofibril; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding

Biological Process: cellular protein complex assembly