NFU1 sirna

NFU1 siRNA (Mouse)

Cat. Num. AAA8221609

• Gene Names: Nfu1; CGI-33; Hirip5; 0610006G17Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NFU1; NFU1 siRNA (Mouse); HIRIP5; NFU1 iron-sulfur cluster scaffold homolog mitochondrial; HIRA-interacting protein 5; mHIRIP5; NFU1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NFU1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 256

• Applicable Applications for NFU1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NFU1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NFU1 sirna

siRNA to inhibit NFU1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 282154803
• NCBI GeneID: 56748
• NCBI Accession #: NP_001164062.1
• NCBI GenBank Nucleotide #: NM_001170591.1
• UniProt Accession #: Q9QZ23; Q3ULE0; Q6VNZ7; Q8CF80; Q9D1B7
• Molecular Weight: 28,568 Da
• NCBI Official Full Name: NFU1 iron-sulfur cluster scaffold homolog, mitochondrial isoform 1
• NCBI Official Synonym Full Names: NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae)
• NCBI Official Symbol: Nfu1
• NCBI Official Synonym Symbols: CGI-33; Hirip5; 0610006G17Rik
• NCBI Protein Information: NFU1 iron-sulfur cluster scaffold homolog, mitochondrial
• UniProt Protein Name: NFU1 iron-sulfur cluster scaffold homolog, mitochondrial
• Protein Family: NifU-like protein
• UniProt Gene Name: Nfu1
• UniProt Synonym Gene Names: Hirip5; mHIRIP5
• UniProt Entry Name: NFU1_MOUSE

Uniprot Description

NFU1: Iron-sulfur cluster scaffold protein which can assemble [4Fe-2S] clusters and deliver them to target proteins. Defects in NFU1 are the cause of multiple mitochondrial dysfunctions syndrome type 1 (MMDS1). A severe disorder of systemic energy metabolism, resulting in weakness, respiratory failure, lack of neurologic development, lactic acidosis, hyperglycinemia and early death. Some patients show failure to thrive, pulmonary hypertension, hypotonia and irritability. Biochemical features include severe combined deficiency of the 2-oxoacid dehydrogenases, defective lipoic acid synthesis and reduction in activity of mitochondrial respiratory chain complexes. Belongs to the NifU family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; iron ion binding; 4 iron, 4 sulfur cluster binding; iron-sulfur cluster binding

Biological Process: iron-sulfur cluster assembly