METRNL sirna

METRNL siRNA (Rat)

Cat. Num. AAA8221421

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: METRNL; METRNL siRNA (Rat); Meteorin-like protein; METRNL sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: METRNL siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 311

• Applicable Applications for METRNL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat METRNL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for METRNL sirna

siRNA to inhibit METRNL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 62078941
• NCBI GeneID: 316842
• NCBI Accession #: NP_001014126.1
• NCBI GenBank Nucleotide #: NM_001014104.1
• UniProt Accession #: Q5RJL6
• Molecular Weight: 34,468 Da
• NCBI Official Full Name: meteorin-like protein
• NCBI Official Synonym Full Names: meteorin, glial cell differentiation regulator-like
• NCBI Official Symbol: Metrnl
• NCBI Protein Information: meteorin-like protein
• UniProt Protein Name: Meteorin-like protein
• Protein Family: Meteorin-like protein
• UniProt Gene Name: Metrnl
• UniProt Entry Name: METRL_RAT

Uniprot Description

Hormone induced following exercise or cold exposure that promotes energy expenditure. Induced either in the skeletal muscle after exercise or in adipose tissue following cold exposure and is present in the circulation. Able to stimulate energy expenditure associated with the browning of the white fat depots and improves glucose tolerance. Does not promote an increase in a thermogenic gene program via direct action on adipocytes, but acts by stimulating several immune cell subtypes to enter the adipose tissue and activate their prothermogenic actions. Stimulates an eosinophil-dependent increase in IL4 expression and promotes alternative activation of adipose tissue macrophages, which are required for the increased expression of the thermogenic and anti-inflammatory gene programs in fat. Required for some cold-induced thermogenic responses, suggesting a role in metabolic adaptations to cold temperatures ().