NFIX sirna

NFIX siRNA (Mouse)

Cat. Num. AAA8221345

• Gene Names: Nfix; CTF; NF1-X; NFI-X; NF-I/X
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NFIX; NFIX siRNA (Mouse); Nuclear factor 1 X-type; NF1-X; Nuclear factor 1/X; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/X; NF-I/X; NFI-X; TGGCA-binding protein; NFIX sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NFIX siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 441

• Applicable Applications for NFIX sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NFIX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NFIX sirna

siRNA to inhibit NFIX expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 126517487
• NCBI GeneID: 18032
• NCBI Accession #: NP_001075451.1
• NCBI GenBank Nucleotide #: NM_001081982.2
• UniProt Accession #: P70257; O08519; P70258; Q64192; Q99L78; Q9R1G2
• Molecular Weight: 44,637 Da
• NCBI Official Full Name: nuclear factor 1 X-type isoform 1
• NCBI Official Synonym Full Names: nuclear factor I/X
• NCBI Official Symbol: Nfix
• NCBI Official Synonym Symbols: CTF; NF1-X; NFI-X; NF-I/X
• NCBI Protein Information: nuclear factor 1 X-type
• UniProt Protein Name: Nuclear factor 1 X-type
• Protein Family: Nuclear factor
• UniProt Gene Name: Nfix
• UniProt Synonym Gene Names: NF1-X; Nuclear factor 1/X; CTF; NF-I/X; NFI-X
• UniProt Entry Name: NFIX_MOUSE

Uniprot Description

NFI-X: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Defects in NFIX are the cause of Sotos syndrome 2 (SOTOS2). A form of Sotos syndrome, a childhood overgrowth syndrome characterized by pre- and postnatal overgrowth, developmental delay, mental retardation, advanced bone age, and abnormal craniofacial morphology. SOTOS2 patients have macrocephaly, long narrow face, high forehead, slender habitus, scoliosis, and unusual behavior characterized especially by anxiety. Defects in NFIX are the cause of Marshall-Smith syndrome (MRSHSS). A distinct malformation syndrome characterized by accelerated skeletal maturation, relative failure to thrive, respiratory difficulties, mental retardation, and unusual facies, including prominent forehead, shallow orbits, blue sclerae, depressed nasal bridge, and micrognathia. Additional skeletal findings include long and thin tubular bones, broad middle phalanges with relatively narrow distal phalanges, and scoliosis. Belongs to the CTF/NF-I family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Cellular Component: intracellular; nucleus

Molecular Function: protein binding; DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; astrocyte differentiation; transcription, DNA-dependent; cerebellar Purkinje cell layer development; cerebellum development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; DNA replication; cerebellar granule cell differentiation