SRSF10 sirna

SRSF10 siRNA (Human)

Cat. Num. AAA8221097

• Gene Names: SRSF10; NSSR; TASR; SRp38; TASR1; TASR2; FUSIP1; FUSIP2; SFRS13; SRrp40; SFRS13A; PPP1R149
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SRSF10; SRSF10 siRNA (Human); FUSIP1; FUSIP2; SFRS13A; TASR; Serine/arginine-rich splicing factor 10; 40 kDa SR-repressor protein; SRrp40; FUS-interacting serine-arginine-rich protein 1; Splicing factor SRp38; Splicing factor. arginine/serine-rich 13A; TLS-associated protein with Ser-Arg repeats; TLS-associated protein with SR repeats; TLS-associated serine-arginine protein; TLS-associated SR protein; SRSF10 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SRSF10 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 182

• Applicable Applications for SRSF10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SRSF10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SRSF10 sirna

siRNA to inhibit SRSF10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 300360547
• NCBI GeneID: 10772
• NCBI Accession #: NP_001177934.1
• NCBI GenBank Nucleotide #: NM_001191005.2
• UniProt Accession #: O75494; O60572; Q5JRH9; Q5JRI0; Q5JRI2; Q5JRI3; Q5JRI4; Q96G09; A6NFM6; A6NI42; A6NIU7; B4DJP9
• Molecular Weight: 22,135 Da
• NCBI Official Full Name: serine/arginine-rich splicing factor 10 isoform 3
• NCBI Official Synonym Full Names: serine/arginine-rich splicing factor 10
• NCBI Official Symbol: SRSF10
• NCBI Official Synonym Symbols: NSSR; TASR; SRp38; TASR1; TASR2; FUSIP1; FUSIP2; SFRS13; SRrp40; SFRS13A; PPP1R149
• NCBI Protein Information: serine/arginine-rich splicing factor 10
• UniProt Protein Name: Serine/arginine-rich splicing factor 10
• Protein Family: Serine/arginine-rich splicing factor
• UniProt Gene Name: SRSF10
• UniProt Synonym Gene Names: FUSIP1; FUSIP2; SFRS13A; TASR; SRrp40; TASR; TLS-associated protein with SR repeats; TLS-associated SR protein
• UniProt Entry Name: SRS10_HUMAN

NCBI Description

This gene product is a member of the serine-arginine (SR) family of proteins, which are involved in constitutive and regulated RNA splicing. Members of this family are characterized by N-terminal RNP1 and RNP2 motifs, which are required for binding to RNA, and multiple C-terminal SR/RS repeats, which are important in mediating association with other cellular proteins. This protein interacts with the oncoprotein TLS, and abrogates the influence of TLS on adenovirus E1A pre-mRNA splicing. This gene has pseudogenes on chromosomes 4, 9, 14, 18, and 20. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

SRSF10: Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. Seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70. Required for splicing repression in M-phase cells and after heat shock. May be involved in regulation of alternative splicing in neurons, with isoform 1 acting as a positive and isoform 3 as a negative regulator. Belongs to the splicing factor SR family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; RS domain binding; RNA binding; unfolded protein binding; nucleotide binding

Biological Process: assembly of spliceosomal tri-snRNP; nuclear mRNA splicing, via spliceosome; RNA splicing, via transesterification reactions; mRNA splice site selection; mRNA export from nucleus; negative regulation of nuclear mRNA splicing, via spliceosome; regulation of transcription, DNA-dependent; cytoplasmic transport