CPSF2 sirna

CPSF2 siRNA (Human)

Cat. Num. AAA8221062

• Gene Names: CPSF2; CPSF100
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CPSF2; CPSF2 siRNA (Human); CPSF100; KIAA1367; Cleavage and polyadenylation specificity factor subunit 2; Cleavage and polyadenylation specificity factor 100 kDa subunit; CPSF 100 kDa subunit; CPSF2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CPSF2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 782

• Applicable Applications for CPSF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CPSF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CPSF2 sirna

siRNA to inhibit CPSF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 34101288
• NCBI GeneID: 53981
• NCBI Accession #: NP_059133.1
• NCBI GenBank Nucleotide #: NM_017437.2
• UniProt Accession #: Q9P2I0; Q6NSJ1; Q9H3W7; B3KME1
• Molecular Weight: 88,487 Da
• NCBI Official Full Name: cleavage and polyadenylation specificity factor subunit 2
• NCBI Official Synonym Full Names: cleavage and polyadenylation specific factor 2, 100kDa
• NCBI Official Symbol: CPSF2
• NCBI Official Synonym Symbols: CPSF100
• NCBI Protein Information: cleavage and polyadenylation specificity factor subunit 2
• UniProt Protein Name: Cleavage and polyadenylation specificity factor subunit 2
• UniProt Gene Name: CPSF2
• UniProt Synonym Gene Names: CPSF100; KIAA1367; CPSF 100 kDa subunit
• UniProt Entry Name: CPSF2_HUMAN

Uniprot Description

CPSF2: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing. Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.

Protein type: RNA splicing

Chromosomal Location of Human Ortholog: 14q31.1

Cellular Component: nucleoplasm; membrane; mRNA cleavage and polyadenylation specificity factor complex

Molecular Function: protein binding; RNA binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; mRNA polyadenylation; gene expression; histone mRNA 3'-end processing; mRNA 3'-end processing; termination of RNA polymerase II transcription; mRNA cleavage