ALG10 sirna

ALG10 siRNA (Human)

Cat. Num. AAA8220812

• Gene Names: ALG10; DIE2; KCR1; ALG10A
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ALG10; ALG10 siRNA (Human); ALG10A; Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1.2-glucosyltransferase; Alpha-1.2-glucosyltransferase ALG10-A; Alpha-2-glucosyltransferase ALG10-A; Asparagine-linked glycosylation protein 10 homolog A; ALG10 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ALG10 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 473

• Applicable Applications for ALG10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ALG10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ALG10 sirna

siRNA to inhibit ALG10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 147904264
• NCBI GeneID: 84920
• NCBI Accession #: NP_116223.3
• NCBI GenBank Nucleotide #: NM_032834.3
• UniProt Accession #: Q5BKT4; Q6NS98; Q96DU0; Q96SM6
• Molecular Weight: 55,606 Da
• NCBI Official Full Name: dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
• NCBI Official Synonym Full Names: ALG10, alpha-1,2-glucosyltransferase
• NCBI Official Symbol: ALG10
• NCBI Official Synonym Symbols: DIE2; KCR1; ALG10A
• NCBI Protein Information: dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
• UniProt Protein Name: Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
• Protein Family: Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
• UniProt Gene Name: ALG10
• UniProt Synonym Gene Names: ALG10A
• UniProt Entry Name: AG10A_HUMAN

NCBI Description

This gene encodes a membrane-associated protein that adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. That is, it transfers the terminal glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol. The rat protein homolog was shown to specifically modulate the gating function of the rat neuronal ether-a-go-go (EAG) potassium ion channel. [provided by RefSeq, Jan 2010]

Uniprot Description

ALG10: Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(2)Man(9)GlcNAc(2)-PP-Dol. Belongs to the ALG10 glucosyltransferase family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transferase; Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.256

Chromosomal Location of Human Ortholog: 12p11.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: transferase activity, transferring hexosyl groups

Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification

Disease: Long Qt Syndrome 2