Rabbit anti-Human ALG10 Polyclonal Antibody | anti-ALG10 antibody

ALG10, NT (ALG10, ALG10A, Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase, Alpha-1,2-glucosyltransferase ALG10-A, Alpha-2-glucosyltransferase ALG10-A, Asparagine-linked glycosylation protein 10 homolog A) (PE)

Cat. Num. AAA6272871

• Gene Names: ALG10; DIE2; KCR1; ALG10A
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ALG10; Polyclonal Antibody; NT (ALG10; ALG10A; Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1; 2-glucosyltransferase; Alpha-1; 2-glucosyltransferase ALG10-A; Alpha-2-glucosyltransferase ALG10-A; Asparagine-linked glycosylation protein 10 homolog A) (PE); Asparagine-linked glycosylation protein 10 homolog A; anti-ALG10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ALG10 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ALG10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23~52 amino acids from the N-terminal region of human ALG10.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ALG10 Antibody (N-term) in A375 cell line lysates (35ug/lane). ALG10 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(ALG10 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALG10 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ALG10 antibody

ALG10 encodes a membrane-associated protein that adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. That is, it transfers the terminal glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol. The rat protein homolog was shown to specifically modulate the gating function of the rat neuronal ether-a-go-go (EAG) potassium ion channel.

Product Categories/Family for anti-ALG10 antibody

Antibodies; Enzymes

References

Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009). Seshadri, S., et al. BMC Med. Genet. 8 SUPPL 1, S15 (2007). Kupershmidt, S., et al. FASEB J. 17(15):2263-2265(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 60551132
• NCBI GeneID: 84920
• UniProt Accession #: Q5BKT4; Q6NS98; Q96DU0; Q96SM6
• Molecular Weight: 55,606 Da
• NCBI Official Full Name: ALG10 protein
• NCBI Official Synonym Full Names: ALG10, alpha-1,2-glucosyltransferase
• NCBI Official Symbol: ALG10
• NCBI Official Synonym Symbols: DIE2; KCR1; ALG10A
• NCBI Protein Information: dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase; dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase; alpha2-glucosyltransferase; potassium channel regulator 1; alpha-2-glucosyltransferase ALG10-A; alpha-1,2-glucosylt
• UniProt Protein Name: Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
• Protein Family: Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
• UniProt Gene Name: ALG10
• UniProt Synonym Gene Names: ALG10A
• UniProt Entry Name: AG10A_HUMAN

NCBI Description

This gene encodes a membrane-associated protein that adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. That is, it transfers the terminal glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol. The rat protein homolog was shown to specifically modulate the gating function of the rat neuronal ether-a-go-go (EAG) potassium ion channel. [provided by RefSeq, Jan 2010]

Uniprot Description

Function: Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol.

Catalytic activity: Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->2)-D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathway: Protein modification; protein glycosylation.

Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein.

Sequence similarities: Belongs to the ALG10 glucosyltransferase family.

Sequence caution: The sequence BAB55272.1 differs from that shown. Reason: Erroneous initiation.