MAGI2 sirna

MAGI2 siRNA (Rat)

Cat. Num. AAA8219352

• Gene Names: Magi2; S-SCAM; Acvrip1; Acvrinp1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAGI2; MAGI2 siRNA (Rat); ACVRINP1; AIP1; SSCAM; Membrane-associated guanylate kinase. WW and PDZ domain-containing protein 2; Atrophin-1-interacting protein 1; AIP-1; Membrane-associated guanylate kinase inverted 2; MAGI-2; Synaptic-scaffolding molecule; S-SCAM; MAGI2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: MAGI2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1277

• Applicable Applications for MAGI2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MAGI2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAGI2 sirna

siRNA to inhibit MAGI2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758422
• NCBI GeneID: 113970
• NCBI Accession #: NP_446073.1
• NCBI GenBank Nucleotide #: NM_053621.1
• UniProt Accession #: O88382; Q9R271
• Molecular Weight: 116,325 Da
• NCBI Official Full Name: membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
• NCBI Official Synonym Full Names: membrane associated guanylate kinase, WW and PDZ domain containing 2
• NCBI Official Symbol: Magi2
• NCBI Official Synonym Symbols: S-SCAM; Acvrip1; Acvrinp1
• NCBI Protein Information: membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
• UniProt Protein Name: Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
• Protein Family: Membrane-associated guanylate kinase, WW and PDZ domain-containing protein
• UniProt Gene Name: Magi2
• UniProt Synonym Gene Names: Acvrinp1; Aip1; Sscam; AIP-1; MAGI-2; S-SCAM
• UniProt Entry Name: MAGI2_RAT

Uniprot Description

AIP1: a scaffold molecule at synaptic junctions that apparently couples neurotransmitter receptors and cell adhesion proteins. Contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. Contains two WW domains, a guanylate kinase-like domain, and multiple PDZ domains. It has structural similarity to the membrane-associated guanylate kinase homologue (MAGUK) family. This protein is brain specific, where it appears to be widely expressed in both neurons and glia. Enhances the ability of PTEN to suppress AKT1 activation. Two splice-variant isoforms have been described.

Protein type: Adaptor/scaffold

Cellular Component: protein complex; tight junction; perinuclear region of cytoplasm; late endosome; cytoplasm; dendrite; postsynaptic density; plasma membrane; synapse; nucleus

Molecular Function: signal transducer activity; protein binding; receptor signaling complex scaffold activity; beta-1 adrenergic receptor binding; protein complex binding; kinase activity; SMAD binding; PDZ domain binding; phosphatase binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; negative regulation of cell proliferation; negative regulation of activin receptor signaling pathway; negative regulation of protein kinase B signaling cascade; protein heterooligomerization; glomerular filtration; positive regulation of receptor internalization; signal transduction; receptor clustering; phosphorylation; negative regulation of cell migration; cytoplasmic transport