TPT1 sirna

TPT1 siRNA (Rat)

Cat. Num. AAA8219247

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TPT1; TPT1 siRNA (Rat); TRT; Translationally-controlled tumor protein; TCTP; Lens epithelial protein; TPT1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: TPT1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 172

• Applicable Applications for TPT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat TPT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TPT1 sirna

siRNA to inhibit TPT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758734
• NCBI GeneID: 116646
• NCBI Accession #: NP_446319.1
• NCBI GenBank Nucleotide #: NM_053867.1
• UniProt Accession #: P63029; P14701
• Molecular Weight: 19,462 Da
• NCBI Official Full Name: translationally-controlled tumor protein
• NCBI Official Synonym Full Names: tumor protein, translationally-controlled 1
• NCBI Official Symbol: Tpt1
• NCBI Protein Information: translationally-controlled tumor protein
• UniProt Protein Name: Translationally-controlled tumor protein
• Protein Family: TPT1-like protein
• UniProt Gene Name: Tpt1
• UniProt Synonym Gene Names: Trt; TCTP
• UniProt Entry Name: TCTP_RAT

NCBI Description

may mediate cell proliferation in normal and tumoral tissues; may play a role in spermatogenesis [RGD, Feb 2006]

Uniprot Description

TPT1: a novel heat shock protein with antiapoptotic and chaperone-like activity. Its levels are highly regulated in response to various cellular stimuli and stresses. Downregulated in response to proapoptotic treatments that induce Ca(++) stress, in a PKR-dependent manner. Colocalizes with CHFR to the mitotic spindle. Appears to be involved in calcium binding and microtubule stabilization. Its exosomal secretion is induced following DNA damage. Interacts with MCL1 and STEAP3, a p53-inducible transmembrane protein that facilitates the exosomal secretion of TPT1. Secreted TPT1 acts as a histamine-releasing factor, participates in inflammatory responses by inducing the release of histamine, and may play a role in tumor reversion. May play a direct role in placental calcium transport. Phosphorylation by Plk may decrease its microtubule-stabilizing activity and promote microtubule dynamics following metaphase. Critical for peripheral T cell maintenance and TCR-mediated cell proliferation.

Protein type: Calcium-binding; Microtubule-binding

Cellular Component: nucleoplasm; multivesicular body; extracellular space; cytoplasm; tubulin complex; nucleus

Molecular Function: calcium ion binding; transcription factor binding

Biological Process: cell proliferation; response to virus; stem cell maintenance; spermatogenesis