PELP1 sirna

PELP1 siRNA (Mouse)

Cat. Num. AAA8218933

• Gene Names: Pelp1; MNAR; 4930563C04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PELP1; PELP1 siRNA (Mouse); MNAR; Proline-. glutamic acid- and leucine-rich protein 1; Modulator of non-genomic activity of estrogen receptor; PELP1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PELP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1123

• Applicable Applications for PELP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PELP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PELP1 sirna

siRNA to inhibit PELP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 257900472
• NCBI GeneID: 75273
• NCBI Accession #: NP_083507.3
• NCBI GenBank Nucleotide #: NM_029231.4
• UniProt Accession #: Q9DBD5; Q5F2E2; Q6PEM0; Q91YM9
• Molecular Weight: 118,069 Da
• NCBI Official Full Name: proline-, glutamic acid- and leucine-rich protein 1
• NCBI Official Synonym Full Names: proline, glutamic acid and leucine rich protein 1
• NCBI Official Symbol: Pelp1
• NCBI Official Synonym Symbols: MNAR; 4930563C04Rik
• NCBI Protein Information: proline-, glutamic acid- and leucine-rich protein 1
• UniProt Protein Name: Proline-, glutamic acid- and leucine-rich protein 1
• Protein Family: Proline-, glutamic acid- and leucine-rich protein
• UniProt Gene Name: Pelp1
• UniProt Synonym Gene Names: Mnar
• UniProt Entry Name: PELP1_MOUSE

Uniprot Description

PELP1: Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence- specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Involved in nuclear receptor signaling via its interaction with AR and NR3C1. May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K. Interacts with HRS, RXRA, SUMO2, HDAC2, RB1 and STAT3. Interacts with PI3K, SRC and EGFR in cytoplasm. Interacts with ESR1 and ESR2 and this interaction is enhanced by 17-beta- estradiol. Interacts with CREBBP, EP300, AR and NR3C1 in a ligand- dependent manner. Forms two complexes in the presence of 17-beta- estradiol; one with SRC and ESR1 and another with LCK and ESR1. Interacts with histone H1 and H3 with a greater affinity for H1. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Isoform 2 is expressed in breast cancer cell lines. Isoform 1 is widely expressed. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus

Molecular Function: chromatin binding; transcription factor binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter