GAPDHS sirna

GAPDHS siRNA (Human)

Cat. Num. AAA8218834

• Gene Names: GAPDHS; GAPD2; GAPDS; HSD-35; GAPDH-2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GAPDHS; GAPDHS siRNA (Human); GAPD2; GAPDH2; GAPDS; Glyceraldehyde-3-phosphate dehydrogenase. testis-specific; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; GAPDH-2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; GAPDHS sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GAPDHS siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 408

• Applicable Applications for GAPDHS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GAPDHS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GAPDHS sirna

siRNA to inhibit GAPDHS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7657116
• NCBI GeneID: 26330
• NCBI Accession #: NP_055179.1
• NCBI GenBank Nucleotide #: NM_014364.4
• UniProt Accession #: O14556; O60823; Q6JTT9; Q9HCU6; B2RC82
• Molecular Weight: 44,501 Da
• NCBI Official Full Name: glyceraldehyde-3-phosphate dehydrogenase, testis-specific
• NCBI Official Synonym Full Names: glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• NCBI Official Symbol: GAPDHS
• NCBI Official Synonym Symbols: GAPD2; GAPDS; HSD-35; GAPDH-2
• NCBI Protein Information: glyceraldehyde-3-phosphate dehydrogenase, testis-specific
• UniProt Protein Name: Glyceraldehyde-3-phosphate dehydrogenase, testis-specific
• Protein Family: Glyceraldehyde-3-phosphate dehydrogenase
• UniProt Gene Name: GAPDHS
• UniProt Synonym Gene Names: GAPD2; GAPDH2; GAPDS; GAPDH-2
• UniProt Entry Name: G3PT_HUMAN

NCBI Description

This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq, Jul 2008]

Uniprot Description

GAPDH-2: May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility. Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Protein type: Oxidoreductase; EC 1.2.1.12

Chromosomal Location of Human Ortholog: 19q13.12

Cellular Component: cytosol; nucleus

Molecular Function: protein binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; NADP binding; NAD binding

Biological Process: sperm motility; glycolysis; carbohydrate metabolic process; positive regulation of glycolysis; glucose metabolic process; pathogenesis; gluconeogenesis