Western Blot (WB)
(Western blot analysis of FAST Kinase expression in PC12 (A), HL60 (B) whole cell lysates.)
Rabbit FAST Kinase Polyclonal Antibody | anti-FASTK antibody
Anti-FAST Kinase Antibody
Western Blot (WB)
(Western blot analysis of FAST Kinase expression in PC12 (A), HL60 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of FAST Kinase expression in PC12 (A), HL60 (B) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of FAST Kinase staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of FAST Kinase staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
FASTK: an atypical protein kinase. Fas-mediated apoptosis is characterized by FASTK- dephosphorylation and TIA-1-phosphorylation. Both are activated and FASTK-mediated TIA-1 activation play a key role in apoptosis.
Protein type: EC 2.7.11.8; Protein kinase, atypical; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; ATYPICAL group; FAST family
Chromosomal Location of Human Ortholog: 7q35
Cellular Component: mitochondrion
Molecular Function: protein serine/threonine kinase activity; protein binding; Fas-activated serine/threonine kinase activity; ATP binding
Biological Process: regulation of RNA splicing; protein amino acid phosphorylation
Research Articles on FASTK
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Product Notes
The FASTK fastk (Catalog #AAA821882) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FAST Kinase Antibody reacts with Human, Rat, Bovine, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's FAST Kinase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC). WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500). Researchers should empirically determine the suitability of the FASTK fastk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAST Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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