REV1 sirna

REV1 siRNA (Human)

Cat. Num. AAA8218747

• Gene Names: REV1; REV1L
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: REV1; REV1 siRNA (Human); REV1L; DNA repair protein REV1; Alpha integrin-binding protein 80; AIBP80; Rev1-like terminal deoxycytidyl transferase; REV1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: REV1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1250

• Applicable Applications for REV1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human REV1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for REV1 sirna

siRNA to inhibit REV1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 84043967
• NCBI GeneID: 51455
• NCBI Accession #: NP_001032961.1
• NCBI GenBank Nucleotide #: NM_001037872.1
• UniProt Accession #: Q9UBZ9; O95941; Q53SI7; Q9C0J4; Q9NUP2
• Molecular Weight: 43,649 Da
• NCBI Official Full Name: DNA repair protein REV1 isoform 2
• NCBI Official Synonym Full Names: REV1, polymerase (DNA directed)
• NCBI Official Symbol: REV1
• NCBI Official Synonym Symbols: REV1L
• NCBI Protein Information: DNA repair protein REV1
• UniProt Protein Name: DNA repair protein REV1
• Protein Family: DNA repair protein
• UniProt Gene Name: REV1
• UniProt Synonym Gene Names: REV1L; AIBP80
• UniProt Entry Name: REV1_HUMAN

NCBI Description

This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found. [provided by RefSeq, Jul 2008]

Uniprot Description

REV1L: Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Belongs to the DNA polymerase type-Y family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; EC 2.7.7.-; Transferase

Chromosomal Location of Human Ortholog: 2q11.1-q11.2

Cellular Component: nucleoplasm

Molecular Function: protein binding; magnesium ion binding; DNA-directed DNA polymerase activity; damaged DNA binding; deoxycytidyl transferase activity

Biological Process: error-prone postreplication DNA repair; DNA replication; DNA repair; response to UV