SUPT5H sirna

SUPT5H siRNA (Human)

Cat. Num. AAA8218520

• Gene Names: SUPT5H; SPT5; SPT5H; Tat-CT1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SUPT5H; SUPT5H siRNA (Human); SPT5; SPT5H; Transcription elongation factor SPT5; hSPT5; DRB sensitivity-inducing factor 160 kDa subunit; DSIF p160; DRB sensitivity-inducing factor large subunit; DSIF large subunit; Tat-cotransactivator 1 protein; Tat-CT1 protein; SUPT5H sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SUPT5H siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1087

• Applicable Applications for SUPT5H sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SUPT5H gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SUPT5H sirna

siRNA to inhibit SUPT5H expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 161169023
• NCBI GeneID: 6829
• NCBI Accession #: NP_001104490.1
• NCBI GenBank Nucleotide #: NM_001111020.2
• UniProt Accession #: O00267; O43279; Q59G52; Q99639
• Molecular Weight: 120,499 Da
• NCBI Official Full Name: transcription elongation factor SPT5 isoform a
• NCBI Official Synonym Full Names: suppressor of Ty 5 homolog (S. cerevisiae)
• NCBI Official Symbol: SUPT5H
• NCBI Official Synonym Symbols: SPT5; SPT5H; Tat-CT1
• NCBI Protein Information: transcription elongation factor SPT5
• UniProt Protein Name: Transcription elongation factor SPT5
• UniProt Gene Name: SUPT5H
• UniProt Synonym Gene Names: SPT5; SPT5H; hSPT5; DSIF p160; DSIF large subunit; Tat-CT1 protein
• UniProt Entry Name: SPT5H_HUMAN

Uniprot Description

SPT5: a transcription elongation factor. Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Two alternatively spliced human isoforms have been described.

Protein type: RNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; enzyme binding; protein heterodimerization activity; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; response to organic substance; chromatin remodeling; mRNA capping; viral reproduction; positive regulation of viral transcription; RNA elongation; positive regulation of RNA elongation; RNA elongation from RNA polymerase II promoter; gene expression; negative regulation of RNA elongation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; cell cycle; positive regulation of macroautophagy