MTTP sirna

MTTP siRNA (Human)

Cat. Num. AAA8218490

• Gene Names: MTTP; ABL; MTP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MTTP; MTTP siRNA (Human); MTP; Microsomal triglyceride transfer protein large subunit; MTTP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MTTP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 894

• Applicable Applications for MTTP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MTTP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MTTP sirna

siRNA to inhibit MTTP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 153285408
• NCBI GeneID: 4547
• NCBI Accession #: NP_000244.2
• NCBI GenBank Nucleotide #: NM_000253.3
• UniProt Accession #: P55157; Q08AM4; Q6P5T3; A8K428
• Molecular Weight: 16,865 Da
• NCBI Official Full Name: microsomal triglyceride transfer protein large subunit isoform 1
• NCBI Official Synonym Full Names: microsomal triglyceride transfer protein
• NCBI Official Symbol: MTTP
• NCBI Official Synonym Symbols: ABL; MTP
• NCBI Protein Information: microsomal triglyceride transfer protein large subunit
• UniProt Protein Name: Microsomal triglyceride transfer protein large subunit
• Protein Family: Microsomal triglyceride transfer protein
• UniProt Gene Name: MTTP
• UniProt Synonym Gene Names: MTP
• UniProt Entry Name: MTP_HUMAN

NCBI Description

MTP encodes the large subunit of the heterodimeric microsomal triglyceride transfer protein. Protein disulfide isomerase (PDI) completes the heterodimeric microsomal triglyceride transfer protein, which has been shown to play a central role in lipoprotein assembly. Mutations in MTP can cause abetalipoproteinemia. [provided by RefSeq, Jul 2008]

Uniprot Description

MTTP: Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B. Defects in MTTP are the cause of abetalipoproteinemia (ABL). ABL is an autosomal recessive disorder of lipoprotein metabolism. Affected individuals produce virtually no circulating apolipoprotein B-containing lipoproteins (chylomicrons, VLDL, LDL, lipoprotein(A)). Malabsorption of the antioxidant vitamin E occurs, leading to spinocerebellar and retinal degeneration.

Protein type: Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: endoplasmic reticulum lumen; receptor complex

Molecular Function: lipid transporter activity; lipid binding

Biological Process: lipoprotein metabolic process; lipid metabolic process; lipid transport

Disease: Abdominal Obesity-metabolic Syndrome 1; Abetalipoproteinemia