MAPK12 sirna

MAPK12 siRNA (Rat)

Cat. Num. AAA8218432

• Gene Names: Mapk12; Sapk3
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAPK12; MAPK12 siRNA (Rat); SAPK3; Mitogen-activated protein kinase 12; MAP kinase 12; MAPK 12; Extracellular signal-regulated kinase 6; ERK-6; Mitogen-activated protein kinase p38 gamma; MAP kinase p38 gamma; Stress-activated protein kinase 3; MAPK12 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: MAPK12 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 367

• Applicable Applications for MAPK12 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MAPK12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAPK12 sirna

siRNA to inhibit MAPK12 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11120692
• NCBI GeneID: 60352
• NCBI Accession #: NP_068514.1
• NCBI GenBank Nucleotide #: NM_021746.1
• UniProt Accession #: Q63538
• Molecular Weight: 41,985 Da
• NCBI Official Full Name: mitogen-activated protein kinase 12
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 12
• NCBI Official Symbol: Mapk12
• NCBI Official Synonym Symbols: Sapk3
• NCBI Protein Information: mitogen-activated protein kinase 12
• UniProt Protein Name: Mitogen-activated protein kinase 12
• UniProt Gene Name: Mapk12
• UniProt Synonym Gene Names: Sapk3; MAP kinase 12; MAPK 12; ERK-6; MAP kinase p38 gamma
• UniProt Entry Name: MK12_RAT

NCBI Description

kinse activated by cellular stresses, bacterial endotoxin and inflammatory cytokines [RGD, Feb 2006]

Uniprot Description

P38G: a proline-directed ser/thr MAP kinase, and one of four p38 kinases that play important roles in cellular responses to inflammatory cytokines, DNA damage, oxidative stress, and some GPCRs. Directly activate transcription factors and of other downstream kinases including MSK1, MSK2, eEF2K, MK2, and PRAK. MSK1 and -2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli. MK2 and -3 control gene expression mostly at the post-transcriptional level. eEF2K is important for the elongation of mRNA during translation. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17, which then cleaves the ectodomain of TGF-alpha family ligands, a process leading to the activation of EGFR signaling and cell proliferation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, CHOPO, p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. Interacts directly with HDAC3 interacts directly and selectively to repress ATF2 transcriptional activity, and regulate TNF gene expression in LPS-stimulated cells. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting that it may inhibit cell proliferation while promoting differentiation. Regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage and G2 arrest after gamma-radiation exposure. Involved in the regulation of GLUT1 expression and basal glucose uptake in L6 myotubes; and negatively regulates GLUT4 expression and contraction-mediated glucose uptake in adult skeletal muscle. C- Jun phosphorylation is stimulated by p38-alpha and inhibited by p38-gamma, leading to a distinct AP-1 regulation. Required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. Positively regulates the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration. Its expression is down-regulation by p38-alpha activation. Highly expressed in skeletal muscle and heart.

Protein type: EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CMGC; CMGC group; MAPK family; p38 subfamily; MAPK/p38 subfamily

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: MAP kinase activity; protein serine/threonine kinase activity; protein binding; magnesium ion binding; ATP binding

Biological Process: myoblast differentiation; peptidyl-serine phosphorylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; MAPKKK cascade; protein amino acid phosphorylation; negative regulation of cell cycle