GDF11 sirna

GDF11 siRNA (Human)

Cat. Num. AAA8218240

• Gene Names: GDF11; BMP11; BMP-11
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GDF11; GDF11 siRNA (Human); BMP11; Growth/differentiation factor 11; GDF-11; Bone morphogenetic protein 11; BMP-11; GDF11 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GDF11 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 407

• Applicable Applications for GDF11 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GDF11 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GDF11 sirna

siRNA to inhibit GDF11 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5031613
• NCBI GeneID: 10220
• NCBI Accession #: NP_005802.1
• NCBI GenBank Nucleotide #: NM_005811.3
• UniProt Accession #: O95390; Q9UID1; Q9UID2
• Molecular Weight: 45,091 Da
• NCBI Official Full Name: growth/differentiation factor 11
• NCBI Official Synonym Full Names: growth differentiation factor 11
• NCBI Official Symbol: GDF11
• NCBI Official Synonym Symbols: BMP11; BMP-11
• NCBI Protein Information: growth/differentiation factor 11
• UniProt Protein Name: Growth/differentiation factor 11
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF11
• UniProt Synonym Gene Names: BMP11; GDF-11; BMP-11
• UniProt Entry Name: GDF11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF11: Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: nervous system development; cell maturation; palate development; regulation of apoptosis; negative regulation of cell proliferation; negative regulation of cell differentiation; camera-type eye morphogenesis; spinal cord anterior/posterior patterning; ureteric bud development; regulation of MAPKKK cascade; pancreas development; mesoderm development; metanephros development; skeletal development; cell development; growth