CXXC1 sirna

CXXC1 siRNA (Human)

Cat. Num. AAA8218206

• Gene Names: CXXC1; CFP1; CGBP; SPP1; PCCX1; PHF18; hCGBP; ZCGPC1; HsT2645; 2410002I16Rik; 5830420C16Rik
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CXXC1; CXXC1 siRNA (Human); CFP1; CGBP; PCCX1; PHF18; CXXC-type zinc finger protein 1; CpG-binding protein; PHD finger and CXXC domain-containing protein 1; CXXC1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CXXC1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 660

• Applicable Applications for CXXC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CXXC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CXXC1 sirna

siRNA to inhibit CXXC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 156142182
• NCBI GeneID: 30827
• NCBI Accession #: NP_001095124.1
• NCBI GenBank Nucleotide #: NM_001101654.1
• UniProt Accession #: Q9P0U4; Q8N2W4; Q96BC8; Q9P2V7; B2RC03
• Molecular Weight: 76,227 Da
• NCBI Official Full Name: CXXC-type zinc finger protein 1 isoform 1
• NCBI Official Synonym Full Names: CXXC finger protein 1
• NCBI Official Symbol: CXXC1
• NCBI Official Synonym Symbols: CFP1; CGBP; SPP1; PCCX1; PHF18; hCGBP; ZCGPC1; HsT2645; 2410002I16Rik; 5830420C16Rik
• NCBI Protein Information: CXXC-type zinc finger protein 1
• UniProt Protein Name: CXXC-type zinc finger protein 1
• Protein Family: CXXC-type zinc finger protein
• UniProt Gene Name: CXXC1
• UniProt Synonym Gene Names: CFP1; CGBP; PCCX1; PHF18
• UniProt Entry Name: CXXC1_HUMAN

NCBI Description

This gene encodes a protein that functions as a transcriptional activator that binds specifically to non-methylated CpG motifs through its CXXC domain. The protein is a component of the SETD1 complex, regulates gene expression and is essential for vertebrate development. [provided by RefSeq, Sep 2015]

Uniprot Description

CXXC1: Transcriptional activator that exhibits a unique DNA binding specificity for CpG unmethylated motifs with a preference for CpGG. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 18q12

Cellular Component: nucleoplasm; histone methyltransferase complex; nuclear matrix; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; zinc ion binding; unmethylated CpG binding; histone lysine N-methyltransferase activity (H3-K4 specific)

Biological Process: unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; regulation of transcription, DNA-dependent; transcription, DNA-dependent; unfolded protein response; positive regulation of transcription, DNA-dependent; histone H3-K4 methylation