F2R sirna

F2R siRNA (Mouse)

Cat. Num. AAA8218033

• Gene Names: F2r; Cf2r; Par1; ThrR; AI482343
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: F2R; F2R siRNA (Mouse); CF2R; PAR1; Proteinase-activated receptor 1; PAR-1; Thrombin receptor; F2R sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: F2R siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 430

• Applicable Applications for F2R sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse F2R gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for F2R sirna

siRNA to inhibit F2R expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22165402
• NCBI GeneID: 14062
• NCBI Accession #: NP_034299.2
• NCBI GenBank Nucleotide #: NM_010169.3
• UniProt Accession #: P30558; P97507; Q3TVP3
• Molecular Weight: 47,790 Da
• NCBI Official Full Name: proteinase-activated receptor 1
• NCBI Official Synonym Full Names: coagulation factor II (thrombin) receptor
• NCBI Official Symbol: F2r
• NCBI Official Synonym Symbols: Cf2r; Par1; ThrR; AI482343
• NCBI Protein Information: proteinase-activated receptor 1
• UniProt Protein Name: Proteinase-activated receptor 1
• Protein Family: Proteinase-activated receptor
• UniProt Gene Name: F2r
• UniProt Synonym Gene Names: Cf2r; Par1; PAR-1
• UniProt Entry Name: PAR1_MOUSE

Uniprot Description

PAR1: a G-protein coupled high-affinity receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelet activation and in vascular development.

Protein type: Receptor, GPCR; Cell development/differentiation; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: postsynaptic membrane; membrane; cell; early endosome; plasma membrane; integral to membrane; caveola; cytosol; neuromuscular junction

Molecular Function: G-protein coupled receptor activity; signal transducer activity; G-protein beta-subunit binding; G-protein alpha-subunit binding; receptor binding; heterotrimeric G-protein binding; thrombin receptor activity

Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of blood coagulation; negative regulation of glomerular filtration; activation of MAPKK activity; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; positive regulation of JAK-STAT cascade; positive regulation of caspase activity; response to lipopolysaccharide; signal transduction; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; connective tissue replacement during inflammatory response; positive regulation of MAPKKK cascade; regulation of interleukin-1 beta production; protein kinase C activation; positive regulation of cell proliferation; response to wounding; positive regulation of smooth muscle contraction; negative regulation of neuron apoptosis; establishment of synaptic specificity at neuromuscular junction; inflammatory response; STAT protein nuclear translocation; caspase activation; platelet activation; death; positive regulation of Rho protein signal transduction; positive regulation of phosphoinositide 3-kinase cascade; tyrosine phosphorylation of STAT protein; G-protein coupled receptor protein signaling pathway; homeostasis of number of cells within a tissue; hemostasis; release of sequestered calcium ion into cytosol; regulation of blood coagulation; positive regulation of vasoconstriction; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of release of sequestered calcium ion into cytosol; regulation of sensory perception of pain; positive regulation of calcium ion transport; blood coagulation; positive regulation of cell migration