UBE2W sirna

UBE2W siRNA (Mouse)

Cat. Num. AAA8217908

• Gene Names: Ube2w; 6130401J04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UBE2W; UBE2W siRNA (Mouse); Ubiquitin-conjugating enzyme E2 W; N-terminus-conjugating E2; Ubiquitin carrier protein W; Ubiquitin-protein ligase W; UBE2W sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: UBE2W siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 151

• Applicable Applications for UBE2W sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse UBE2W gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UBE2W sirna

siRNA to inhibit UBE2W expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 401891882
• NCBI GeneID: 66799
• NCBI Accession #: NP_080049.2
• NCBI GenBank Nucleotide #: NM_025773.3
• UniProt Accession #: Q8VDW4; Q8BVJ8; Q9D5H3
• Molecular Weight: 17,345 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 W isoform 1
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2W (putative)
• NCBI Official Symbol: Ube2w
• NCBI Official Synonym Symbols: 6130401J04Rik
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 W
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 W
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: Ube2w
• UniProt Entry Name: UBE2W_MOUSE

Uniprot Description

UBE2W: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; EC 6.3.2.19; Enzyme, misc.; Ubiquitin ligase; Ubiquitin conjugating system

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; nucleotide binding; ATP binding; ligase activity

Biological Process: protein monoubiquitination; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; misfolded or incompletely synthesized protein catabolic process; DNA repair; response to DNA damage stimulus