USP22 sirna

USP22 siRNA (Mouse)

Cat. Num. AAA8217801

• Gene Names: Usp22; AI427806
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: USP22; USP22 siRNA (Mouse); KIAA1063; Ubiquitin carboxyl-terminal hydrolase 22; Deubiquitinating enzyme 22; Ubiquitin thioesterase 22; Ubiquitin-specific-processing protease 22; USP22 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: USP22 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 525

• Applicable Applications for USP22 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse USP22 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for USP22 sirna

siRNA to inhibit USP22 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 110625685
• NCBI GeneID: 216825
• NCBI Accession #: NP_001004143.2
• NCBI GenBank Nucleotide #: NM_001004143.4
• UniProt Accession #: Q5DU02; Q3TU34; Q3U2W4; Q5SU81; Q66JV8; Q6PDX3; Q8BJG3
• Molecular Weight: 59,954 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 22
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 22
• NCBI Official Symbol: Usp22
• NCBI Official Synonym Symbols: AI427806
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 22
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 22
• UniProt Gene Name: Usp22
• UniProt Synonym Gene Names: Kiaa1063
• UniProt Entry Name: UBP22_MOUSE

Uniprot Description

USP22: Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression. Belongs to the peptidase C19 family. UBP8 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Transcription, coactivator/corepressor; Ubiquitin-specific protease; Nuclear receptor co-regulator; Protease; Ubiquitin conjugating system; EC 3.4.19.12

Cellular Component: nucleus

Molecular Function: peptidase activity; H4 histone acetyltransferase activity; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; hydrolase activity; zinc ion binding; cysteine-type endopeptidase activity; metal ion binding; ubiquitin-specific protease activity; transcription coactivator activity; cysteine-type peptidase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone ubiquitination; chromatin modification; proteolysis; cell cycle; positive regulation of mitotic cell cycle; histone deubiquitination