IER3IP1 sirna

IER3IP1 siRNA (Human)

Cat. Num. AAA8217119

• Gene Names: IER3IP1; MEDS; HSPC039; PRO2309
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IER3IP1; IER3IP1 siRNA (Human); Immediate early response 3-interacting protein 1; IER3IP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: IER3IP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 82

• Applicable Applications for IER3IP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IER3IP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IER3IP1 sirna

siRNA to inhibit IER3IP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7705819
• NCBI GeneID: 51124
• NCBI Accession #: NP_057181.1
• NCBI GenBank Nucleotide #: NM_016097.4
• UniProt Accession #: Q9Y5U9
• Molecular Weight: 8,969 Da
• NCBI Official Full Name: immediate early response 3-interacting protein 1
• NCBI Official Synonym Full Names: immediate early response 3 interacting protein 1
• NCBI Official Symbol: IER3IP1
• NCBI Official Synonym Symbols: MEDS; HSPC039; PRO2309
• NCBI Protein Information: immediate early response 3-interacting protein 1
• UniProt Protein Name: Immediate early response 3-interacting protein 1
• Protein Family: Immediate early response 3-interacting protein
• UniProt Gene Name: IER3IP1
• UniProt Entry Name: IR3IP_HUMAN

NCBI Description

This gene encodes a small protein that is localized to the endoplasmic reticulum (ER) and may play a role in the ER stress response by mediating cell differentiation and apoptosis. Transcription of this gene is regulated by tumor necrosis factor alpha and specificity protein 1 (Sp1). Mutations in this gene may play a role in microcephaly, epilepsy, and diabetes syndrome (MEDS), and a pseudogene of this gene is located on the long arm of chromosome 12. [provided by RefSeq, Dec 2011]

Uniprot Description

IER3IP1: May be implicated in the regulation of apoptosis. May be involved in protein transport between endoplasmic reticulum and Golgi apparatus. Defects in IER3IP1 are the cause of microcephaly epilepsy and diabetes syndrome (MEDS). An autosomal recessive disorder characterized by microcephaly, simplified gyral pattern, severe epilepsy, and infantile diabetes. Belongs to the YOS1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q12

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Disease: Microcephaly, Epilepsy, And Diabetes Syndrome