SF3B4 sirna

SF3B4 siRNA (Human)

Cat. Num. AAA8217001

• Gene Names: SF3B4; AFD1; Hsh49; SAP49; SF3b49
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SF3B4; SF3B4 siRNA (Human); SAP49; Splicing factor 3B subunit 4; Pre-mRNA-splicing factor SF3b 49 kDa subunit; SF3b50; Spliceosome-associated protein 49; SAP 49; SF3B4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SF3B4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 424

• Applicable Applications for SF3B4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SF3B4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SF3B4 sirna

siRNA to inhibit SF3B4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5032069
• NCBI GeneID: 10262
• NCBI Accession #: NP_005841.1
• NCBI GenBank Nucleotide #: NM_005850.4
• UniProt Accession #: Q15427; Q5SZ63
• Molecular Weight: 44,386 Da
• NCBI Official Full Name: splicing factor 3B subunit 4
• NCBI Official Synonym Full Names: splicing factor 3b, subunit 4, 49kDa
• NCBI Official Symbol: SF3B4
• NCBI Official Synonym Symbols: AFD1; Hsh49; SAP49; SF3b49
• NCBI Protein Information: splicing factor 3B subunit 4
• UniProt Protein Name: Splicing factor 3B subunit 4
• Protein Family: Splicing factor
• UniProt Gene Name: SF3B4
• UniProt Synonym Gene Names: SAP49; SAP 49
• UniProt Entry Name: SF3B4_HUMAN

NCBI Description

This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq, Jul 2008]

Uniprot Description

SF3B4: subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Protein type: RNA-binding; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: nucleoplasm; spliceosome; U12-dependent spliceosome

Molecular Function: protein binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of nuclear mRNA splicing, via spliceosome; RNA splicing, via transesterification reactions; RNA splicing; gene expression; mRNA processing

Disease: Acrofacial Dysostosis 1, Nager Type