PSIP1 sirna

PSIP1 siRNA (Human)

Cat. Num. AAA8216932

• Gene Names: PSIP1; p52; p75; PAIP; DFS70; LEDGF; PSIP2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PSIP1; PSIP1 siRNA (Human); DFS70; LEDGF; PSIP2; PC4 and SFRS1-interacting protein; CLL-associated antigen KW-7; Dense fine speckles 70 kDa protein; DFS 70; Lens epithelium-derived growth factor; Transcriptional coactivator p75/p52; PSIP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PSIP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 329

• Applicable Applications for PSIP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PSIP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PSIP1 sirna

siRNA to inhibit PSIP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 190014588
• NCBI GeneID: 11168
• NCBI Accession #: NP_001121689.1
• NCBI GenBank Nucleotide #: NM_001128217.1
• UniProt Accession #: O75475; O00256; O95368; Q6P391; Q86YB9; Q9NZI3; Q9UER6; D3DRI9
• Molecular Weight: 37,268 Da
• NCBI Official Full Name: PC4 and SFRS1-interacting protein isoform 2
• NCBI Official Synonym Full Names: PC4 and SFRS1 interacting protein 1
• NCBI Official Symbol: PSIP1
• NCBI Official Synonym Symbols: p52; p75; PAIP; DFS70; LEDGF; PSIP2
• NCBI Protein Information: PC4 and SFRS1-interacting protein
• UniProt Protein Name: PC4 and SFRS1-interacting protein
• Protein Family: PC4 and SFRS1-interacting protein
• UniProt Gene Name: PSIP1
• UniProt Synonym Gene Names: DFS70; LEDGF; PSIP2; DFS 70
• UniProt Entry Name: PSIP1_HUMAN

Uniprot Description

LEDGF: Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration. Monomer. Interacts with IFRD1/PC4. Isoform 2 interacts with SFRS1. Isoform 1 interacts POGZ and CDCA7L. Isoform 1 interacts with lentiviral integrase and acts as a chromatin tethering factor to the chromosomal DNA. Isoform 1 interacts in particular with the human HIV-1 integrase protein (HIV-1 IN), determining its nuclear localization, its tight association with chromatin and its protection from the proteasome. Isoform 1 interacts also with HIV-2 IN. Isoform 2 does not interact with HIV-1 IN. Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions. Belongs to the HDGF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9p22.3

Cellular Component: nucleoplasm; nucleolus; nuclear heterochromatin; cytosol; nucleus

Molecular Function: protein binding; transcription activator binding; chromatin binding

Biological Process: nuclear mRNA 5'-splice site recognition; regulation of transcription, DNA-dependent; transcription, DNA-dependent; viral reproduction; response to heat; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress