SMARCAD1 sirna

SMARCAD1 siRNA (Human)

Cat. Num. AAA8216776

• Gene Names: SMARCAD1; ETL1; HEL1; ADERM
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SMARCAD1; SMARCAD1 siRNA (Human); KIAA1122; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1; ATP-dependent helicase 1; hHEL1; SMARCAD1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SMARCAD1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 596

• Applicable Applications for SMARCAD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SMARCAD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SMARCAD1 sirna

siRNA to inhibit SMARCAD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 190358532
• NCBI GeneID: 56916
• NCBI Accession #: NP_001121901.1
• NCBI GenBank Nucleotide #: NM_001128429.2
• UniProt Accession #: Q9H4L7; Q05D56; Q96SX1; Q9H017; Q9H860; Q9NPU9; Q9ULU7; B7Z799
• Molecular Weight: 68,774 Da
• NCBI Official Full Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 isoform a
• NCBI Official Synonym Full Names: SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1
• NCBI Official Symbol: SMARCAD1
• NCBI Official Synonym Symbols: ETL1; HEL1; ADERM
• NCBI Protein Information: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1
• UniProt Protein Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1
• Protein Family: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily
• UniProt Gene Name: SMARCAD1
• UniProt Synonym Gene Names: KIAA1122; hHEL1
• UniProt Entry Name: SMRCD_HUMAN

NCBI Description

This gene encodes a member of the SNF subfamily of helicase proteins. The encoded protein plays a critical role in the restoration of heterochromatin organization and propagation of epigenetic patterns following DNA replication by mediating histone H3/H4 deacetylation. Mutations in this gene are associated with adermatoglyphia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

SMARCAD1: an ubiquitous enzyme with ATP-dependent DNA helicase activity. Belongs to the SNF2/RAD54 helicase family. Two alternatively spliced human isoforms have been described.

Protein type: EC 3.6.4.12; Adaptor/scaffold; Helicase

Chromosomal Location of Human Ortholog: 4q22.3

Cellular Component: nucleoplasm; heterochromatin; nuclear matrix; nuclear replication fork

Molecular Function: protein binding; nucleic acid binding; DNA binding; helicase activity; ATP binding

Biological Process: chromatin remodeling; positive regulation of transcription, DNA-dependent; regulation of DNA recombination; DNA double-strand break processing; ATP-dependent chromatin remodeling; nucleotide metabolic process; chromatin modification; chromosome separation; protein homooligomerization

Disease: Adermatoglyphia