BRIP1 sirna

BRIP1 siRNA (Mouse)

Cat. Num. AAA8216296

• Gene Names: Brip1; OF; FACJ; Bach1; Fancj; 3110009N10Rik; 8030460J03Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BRIP1; BRIP1 siRNA (Mouse); BACH1; FANCJ; Fanconi anemia group J protein homolog; Protein FACJ; ATP-dependent RNA helicase BRIP1; BRCA1-associated C-terminal helicase 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1-interacting protein 1; BRIP1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BRIP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,174

• Applicable Applications for BRIP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BRIP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BRIP1 sirna

siRNA to inhibit BRIP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 30795235
• NCBI GeneID: 237911
• NCBI Accession #: NP_840094.1
• NCBI GenBank Nucleotide #: NM_178309.2
• UniProt Accession #: Q5SXJ3; Q8BJQ8; Q8BKI6
• Molecular Weight: 131,360 Da
• NCBI Official Full Name: Fanconi anemia group J protein homolog
• NCBI Official Synonym Full Names: BRCA1 interacting protein C-terminal helicase 1
• NCBI Official Symbol: Brip1
• NCBI Official Synonym Symbols: OF; FACJ; Bach1; Fancj; 3110009N10Rik; 8030460J03Rik
• NCBI Protein Information: Fanconi anemia group J protein homolog
• UniProt Protein Name: Fanconi anemia group J protein homolog
• Protein Family: Fanconi anemia group J protein
• UniProt Gene Name: Brip1
• UniProt Synonym Gene Names: Bach1; Fancj; Protein FACJ; BRCA1-interacting protein 1
• UniProt Entry Name: FANCJ_MOUSE

NCBI Description

This gene encodes a member of the DEAH subfamily of DEAD box helicases. A similar protein in humans is both a DNA-dependent ATPase and a 5-prime-to-3-prime DNA helicase, and plays a role in the repair of DNA double stranded breaks through interaction with the breast cancer-associated tumor suppressor BRCA1. [provided by RefSeq, Feb 2011]

Uniprot Description

BRIP1: a DNA-dependent ATPase and DNA helicase required for the maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. Binds directly to the BRCT domains of BRCA1. Defects in BRIP1 cause of susceptibility to breast cancer and Fanconi anemia. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Belongs to the DEAD box helicase family, DEAH subfamily. Two alternatively spliced human isoforms have been described.

Protein type: EC 3.6.4.13; Helicase; Oncoprotein

Cellular Component: nuclear membrane; cytoplasm; nucleus

Molecular Function: ATP-dependent DNA helicase activity; DNA binding; nucleic acid binding; hydrolase activity; 4 iron, 4 sulfur cluster binding; metal ion binding; iron-sulfur cluster binding; nucleotide binding; ATP-dependent helicase activity; helicase activity; ATP binding; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides

Biological Process: regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; DNA repair; response to DNA damage stimulus