CNTN1 sirna

CNTN1 siRNA (Mouse)

Cat. Num. AAA8216206

• Gene Names: Cntn1; usl; CNTN; F3cam; AW495098
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CNTN1; CNTN1 siRNA (Mouse); Contactin-1; Neural cell surface protein F3; CNTN1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CNTN1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,020

• Applicable Applications for CNTN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CNTN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CNTN1 sirna

siRNA to inhibit CNTN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 227497224
• NCBI GeneID: 12805
• NCBI Accession #: NP_001153119.1
• NCBI GenBank Nucleotide #: NM_001159647.1
• UniProt Accession #: P12960; Q6NXV7; Q8BR42; Q8C6A0
• Molecular Weight: 113,388 Da
• NCBI Official Full Name: contactin-1
• NCBI Official Synonym Full Names: contactin 1
• NCBI Official Symbol: Cntn1
• NCBI Official Synonym Symbols: usl; CNTN; F3cam; AW495098
• NCBI Protein Information: contactin-1
• UniProt Protein Name: Contactin-1
• Protein Family: Contactin
• UniProt Gene Name: Cntn1
• UniProt Entry Name: CNTN1_MOUSE

Uniprot Description

CNTN1: Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. Defects in CNTN1 are the cause of Compton-North congenital myopathy (CNCM). CNCM is a familial lethal form of congenital onset muscle weakness, inherited in an autosomal-recessive fashion and characterized by a secondary loss of beta2-syntrophin and alpha-dystrobrevin from the muscle sarcolemma, central nervous system involvement, and fetal akinesia. Belongs to the immunoglobulin superfamily. Contactin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor

Cellular Component: membrane; plasma membrane; myelin sheath; lipid raft

Molecular Function: protein binding; carbohydrate binding; glycoprotein binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; Notch signaling pathway; cerebellum development; cell adhesion; neurite development