PXDN sirna

PXDN siRNA (Human)

Cat. Num. AAA8216027

• Gene Names: PXDN; PXN; VPO; MG50; PRG2; COPOA; D2S448; D2S448E
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PXDN; PXDN siRNA (Human); KIAA0230; MG50; PRG2; VPO; VPO1; Peroxidasin homolog; Melanoma-associated antigen MG50; Vascular peroxidase 1; p53-responsive gene 2 protein; PXDN sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PXDN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 727

• Applicable Applications for PXDN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PXDN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PXDN sirna

siRNA to inhibit PXDN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 109150416
• NCBI GeneID: 7837
• NCBI Accession #: NP_036425.1
• NCBI GenBank Nucleotide #: NM_012293.2
• UniProt Accession #: Q92626; Q4KMG2; A8QM65; D6W4Y0
• Molecular Weight: 80,329 Da
• NCBI Official Full Name: peroxidasin homolog
• NCBI Official Synonym Full Names: peroxidasin
• NCBI Official Symbol: PXDN
• NCBI Official Synonym Symbols: PXN; VPO; MG50; PRG2; COPOA; D2S448; D2S448E
• NCBI Protein Information: peroxidasin homolog
• UniProt Protein Name: Peroxidasin homolog
• UniProt Gene Name: PXDN
• UniProt Synonym Gene Names: KIAA0230; MG50; PRG2; VPO; VPO1
• UniProt Entry Name: PXDN_HUMAN

NCBI Description

This gene encodes a heme-containing peroxidase that is secreted into the extracellular matrix. It is involved in extracellular matrix formation, and may function in the physiological and pathological fibrogenic response in fibrotic kidney. Mutations in this gene cause corneal opacification and other ocular anomalies, and also microphthalmia and anterior segment dysgenesis. [provided by RefSeq, Aug 2014]

Uniprot Description

PXDN: Displays low peroxidase activity and is likely to participate in H(2)O(2) metabolism and peroxidative reactions in the cardiovascular system. Plays a role in extracellular matrix formation. Belongs to the peroxidase family. XPO subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; EC 1.11.1.7; Oxidoreductase

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; endoplasmic reticulum

Molecular Function: peroxidase activity; metal ion binding; extracellular matrix structural constituent; heme binding; interleukin-1 receptor antagonist activity

Biological Process: extracellular matrix organization and biogenesis; hydrogen peroxide catabolic process; immune response; negative regulation of cytokine and chemokine mediated signaling pathway; response to oxidative stress

Disease: Corneal Opacification With Other Ocular Anomalies