DMRT1 sirna

DMRT1 siRNA (Human)

Cat. Num. AAA8215752

• Gene Names: DMRT1; DMT1; CT154
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DMRT1; DMRT1 siRNA (Human); DMT1; Doublesex- and mab-3-related transcription factor 1; DM domain expressed in testis protein 1; DMRT1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DMRT1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 373

• Applicable Applications for DMRT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DMRT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DMRT1 sirna

siRNA to inhibit DMRT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 62241026
• NCBI GeneID: 1761
• NCBI Accession #: NP_068770.2
• NCBI GenBank Nucleotide #: NM_021951.2
• UniProt Accession #: Q9Y5R6; Q6T1H8; Q6T1H9; Q8IW77; B2R913
• Molecular Weight: 18,325 Da
• NCBI Official Full Name: doublesex- and mab-3-related transcription factor 1
• NCBI Official Synonym Full Names: doublesex and mab-3 related transcription factor 1
• NCBI Official Symbol: DMRT1
• NCBI Official Synonym Symbols: DMT1; CT154
• NCBI Protein Information: doublesex- and mab-3-related transcription factor 1
• UniProt Protein Name: Doublesex- and mab-3-related transcription factor 1
• Protein Family: Doublesex and mab-3 related transcription factor
• UniProt Gene Name: DMRT1
• UniProt Synonym Gene Names: DMT1
• UniProt Entry Name: DMRT1_HUMAN

NCBI Description

This gene is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous. [provided by RefSeq, Jul 2008]

Uniprot Description

DMRT1: Transcription factor that plays a key role in male sex determination and differentiation by controlling testis development and male germ cell proliferation. Plays a central role in spermatogonia by inhibiting meiosis in undifferentiated spermatogonia and promoting mitosis, leading to spermatogonial development and allowing abundant and continuous production of sperm. Acts both as a transcription repressor and activator: prevents meiosis by restricting retinoic acid (RA)-dependent transcription and repressing STRA8 expression and promotes spermatogonial development by activating spermatogonial differentiation genes, such as SOHLH1. Also plays a key role in postnatal sex maintenance by maintaining testis determination and preventing feminization: represses transcription of female promoting genes such as FOXL2 and activates male-specific genes. May act as a tumor suppressor. May also play a minor role in oogenesis. Defects in DMRT1 may be a cause of testicular germ cell tumor (TGCT). A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms. Defects in DMRT1 may be a cause of 46,XY sex reversal type 4 (SRXY4). A condition characterized by male-to- female sex reversal in the presence of a normal 46,XY karyotype. Patients display complete or partial gonadal dysgenesis and a chromosome 9p deletion. Belongs to the DMRT family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 9p24.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein homodimerization activity; protein heterodimerization activity; metal ion binding; chromatin binding; transcription factor activity

Biological Process: positive regulation of mitosis; transcription, DNA-dependent; cell morphogenesis; Sertoli cell differentiation; negative regulation of transcription from RNA polymerase II promoter; Sertoli cell development; male sex differentiation; male sex determination; germ cell migration; negative regulation of meiosis; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; oocyte development