Human Neurofibromin ELISA Kit | NF1 elisa kit

Human Neurofibromin ELISA Kit

Cat. Num. AAA763921

• Reactivity: Human
• Synonyms: Neurofibromin; Human Neurofibromin ELISA Kit; NF1/Neurofibromatosis-related protein NF-1; NF1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NF1. No significant cross-reactivity or interference between NF1 and analogues was observed.
• Sequence Length: 2820

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NF1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 6981264
• NCBI GeneID: 24592
• NCBI Accession #: NP_036741.1
• NCBI GenBank Nucleotide #: NM_012609.1
• Molecular Weight: 317,083 Da
• NCBI Official Full Name: neurofibromin
• NCBI Official Synonym Full Names: neurofibromin 1
• NCBI Official Symbol: Nf1
• NCBI Protein Information: neurofibromin
• UniProt Protein Name: Neurofibromin
• Protein Family: Neurofibromin
• UniProt Gene Name: Nf1
• UniProt Entry Name: NF1_RAT

NCBI Description

mutations in gene are associated with neurofibromatosis type 1 [RGD, Feb 2006]

Uniprot Description

NF1: a Ras-GAP, highly expressed in developing neural cells. Possesses tumor suppressor activity, presumably by virtue of its GTPase activating domain. Neurofibromin is phosphorylated in response to EGF in CNS cells and cell lines. Defects in NF1 are the cause of type 1 neurofibromatosis (NF1), Watson syndrome, and familial spinal neurofibromatosis. NF1 is one of the most frequent autosomal dominant diseases. Four alternatively spliced isoforms have been described.

Protein type: GAPs, Ras; Tumor suppressor; GAPs; Nucleolus; Motility/polarity/chemotaxis

Cellular Component: axon; cytoplasm; dendrite; membrane; neuron projection; nucleolus; nucleus; postsynaptic density; protein complex

Molecular Function: GTPase activator activity; microtubule binding; phosphatidylcholine binding; phosphatidylethanolamine binding; protein binding; syndecan binding

Biological Process: actin cytoskeleton organization and biogenesis; adrenal gland development; amygdala development; artery morphogenesis; brain development; camera-type eye morphogenesis; cell communication; cerebral cortex development; cognition; collagen fibril organization; extracellular matrix organization and biogenesis; forebrain astrocyte development; forebrain morphogenesis; heart development; induction of apoptosis via death domain receptors; liver development; MAPKKK cascade; metanephros development; myelination in the peripheral nervous system; negative regulation of angiogenesis; negative regulation of astrocyte differentiation; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of cell-matrix adhesion; negative regulation of endothelial cell proliferation; negative regulation of fibroblast proliferation; negative regulation of MAP kinase activity; negative regulation of MAPKKK cascade; negative regulation of neuroblast proliferation; negative regulation of neurotransmitter secretion; negative regulation of oligodendrocyte differentiation; negative regulation of osteoclast differentiation; negative regulation of protein kinase activity; negative regulation of Rac protein signal transduction; negative regulation of Ras protein signal transduction; negative regulation of transcription factor import into nucleus; neural tube development; osteoblast differentiation; peripheral nervous system development; phosphoinositide 3-kinase cascade; pigmentation; positive regulation of adenylate cyclase activity; positive regulation of apoptosis; positive regulation of endothelial cell proliferation; positive regulation of GTPase activity; positive regulation of neuron apoptosis; Ras protein signal transduction; regulation of angiogenesis; regulation of blood vessel endothelial cell migration; regulation of bone resorption; regulation of cell proliferation; regulation of cell-matrix adhesion; regulation of gene expression; regulation of glial cell differentiation; regulation of GTPase activity; regulation of long-term neuronal synaptic plasticity; regulation of MAPKKK cascade; regulation of neuron differentiation; regulation of synaptic transmission, GABAergic; response to hypoxia; Schwann cell development; signal transduction; skeletal muscle development; smooth muscle development; spinal cord development; sympathetic nervous system development; visual learning; wound healing