UXT sirna

UXT siRNA (Human)

Cat. Num. AAA8215736

• Gene Names: UXT; STAP1; ART-27
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UXT; UXT siRNA (Human); Protein UXT; Androgen receptor trapped clone 27 protein; ART-27; Ubiquitously expressed transcript protein; UXT sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: UXT siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 157

• Applicable Applications for UXT sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human UXT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UXT sirna

siRNA to inhibit UXT expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4759298
• NCBI GeneID: 8409
• NCBI Accession #: NP_004173.1
• NCBI GenBank Nucleotide #: NM_004182.3
• UniProt Accession #: Q9UBK9; Q5JZG3; Q9Y6E5; B2R561
• Molecular Weight: 18,246 Da
• NCBI Official Full Name: protein UXT isoform 2
• NCBI Official Synonym Full Names: ubiquitously-expressed, prefoldin-like chaperone
• NCBI Official Symbol: UXT
• NCBI Official Synonym Symbols: STAP1; ART-27
• NCBI Protein Information: protein UXT
• UniProt Protein Name: Protein UXT
• Protein Family: Protein
• UniProt Gene Name: UXT
• UniProt Synonym Gene Names: ART-27
• UniProt Entry Name: UXT_HUMAN

NCBI Description

The protein encoded by this gene functions as a cofactor that modulates androgen receptor-dependent transcription, and also plays a critical role in tumor necrosis factor-induced apoptosis. Expression of this gene may play a role in tumorigenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

UXT: Involved in gene transcription regulation. Acts in concert with the corepressor URI1 to regulate androgen receptor transcription (AR). AR N-terminus-associated coactivator which may play a role in facilitating receptor-induced transcriptional activation (PubMed:11854421). Potential component of mitochondrial-associated LRPPRC, a multidomain organizer that potentially integrates mitochondria and the microtubular cytoskeleton with chromosome remodeling (PubMed:11827465). Increasing concentrations of UXT contributes to progressive aggregation of mitochondria and cell death potentially through its association with LRPPRC (PubMed:17554592). May be a nuclear chaperone that promotes formation of the NF-kappa-B enhanceosome and which is essential for its nuclear function (PubMed:17620405). Suppresses cell transformation and it might mediate this function by interaction and inhibition of the biological activity of cell proliferation and survival stimulatory factors like MECOM (PubMed:17635584). Together with URI1, associates with chromatin to the NKX3-1 promoter region. Belongs to the UXT family.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: Xp11.23-p11.22

Cellular Component: centrosome; cytoskeleton; cytoplasm; nucleus; gamma-tubulin complex

Molecular Function: actin filament binding; protein binding; microtubule binding; beta-tubulin binding; chromatin binding

Biological Process: transcription, DNA-dependent; centrosome organization and biogenesis; negative regulation of transcription from RNA polymerase II promoter; microtubule cytoskeleton organization and biogenesis; mitochondrion transport along microtubule