ARHGAP26 sirna

ARHGAP26 siRNA (Human)

Cat. Num. AAA8215720

• Gene Names: ARHGAP26; GRAF; GRAF1; OPHN1L; OPHN1L1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ARHGAP26; ARHGAP26 siRNA (Human); GRAF; KIAA0621; OPHN1L; Rho GTPase-activating protein 26; GTPase regulator associated with focal adhesion kinase; Oligophrenin-1-like protein; Rho-type GTPase-activating protein 26; ARHGAP26 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ARHGAP26 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 759

• Applicable Applications for ARHGAP26 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ARHGAP26 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ARHGAP26 sirna

siRNA to inhibit ARHGAP26 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 208022687
• NCBI GeneID: 23092
• NCBI Accession #: NP_001129080.1
• NCBI GenBank Nucleotide #: NM_001135608.1
• UniProt Accession #: Q9UNA1; O75117; Q5D035; Q9BYS6; Q9BYS7; Q9UJ00
• Molecular Weight: 86,165 Da
• NCBI Official Full Name: rho GTPase-activating protein 26 isoform b
• NCBI Official Synonym Full Names: Rho GTPase activating protein 26
• NCBI Official Symbol: ARHGAP26
• NCBI Official Synonym Symbols: GRAF; GRAF1; OPHN1L; OPHN1L1
• NCBI Protein Information: rho GTPase-activating protein 26
• UniProt Protein Name: Rho GTPase-activating protein 26
• Protein Family: Rho GTPase-activating protein
• UniProt Gene Name: ARHGAP26
• UniProt Synonym Gene Names: GRAF; KIAA0621; OPHN1L
• UniProt Entry Name: RHG26_HUMAN

NCBI Description

Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. The protein encoded by this gene is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc42. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

ARHGAP26: GTPase-activating protein for RHOA and CDC42. Interacts with NYAP1, NYAP2 and MYO16. Binds to the C-terminus of PTK2/FAK1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; GAPs, Rac/Rho; Motility/polarity/chemotaxis; GAPs

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: focal adhesion; cytoskeleton; cytosol

Molecular Function: protein binding; phospholipid binding

Biological Process: nervous system development; regulation of small GTPase mediated signal transduction; plasma membrane organization and biogenesis; small GTPase mediated signal transduction; actin cytoskeleton organization and biogenesis

Disease: Juvenile Myelomonocytic Leukemia