APITD1 sirna

APITD1 siRNA (Human)

Cat. Num. AAA8215696

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APITD1; APITD1 siRNA (Human); CENPS; FAAP16; MHF1; Centromere protein S; CENP-S; Apoptosis-inducing TAF9-like domain-containing protein 1; FANCM-interacting histone fold protein 1; Fanconi anemia-associated polypeptide of 16 kDa; APITD1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: APITD1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 138

• Applicable Applications for APITD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human APITD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APITD1 sirna

siRNA to inhibit APITD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 395394019
• NCBI GeneID: 100526739
• NCBI Accession #: NP_001257446.1
• NCBI GenBank Nucleotide #: NM_001270517.1
• UniProt Accession #: Q8N2Z9; Q8NFE5; Q8NFG5
• Molecular Weight: 9,074 Da
• NCBI Official Full Name: APITD1-CORT protein isoform 4
• NCBI Official Synonym Full Names: APITD1-CORT readthrough
• NCBI Official Symbol: APITD1-CORT
• NCBI Protein Information: APITD1-CORT protein
• UniProt Protein Name: Centromere protein S
• Protein Family: Centromere protein
• UniProt Gene Name: APITD1
• UniProt Synonym Gene Names: CENPS; FAAP16; MHF1; CENP-S
• UniProt Entry Name: CENPS_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring APITD1 (apoptosis-inducing, TAF9-like domain 1) and CORT (cortistatin) genes. Alternative splicing results in multiple transcript variants, two of which encode fusion proteins that share sequence identity with the products of each individual gene. [provided by RefSeq, Aug 2011]

Uniprot Description

APITD1: DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance. Required for optimal chromatin association of the FA core complex. Required for efficient damage-induced monoubiquitination and focus formation of FANCD2. Stabilizes FAAD24, FANCM and STRA13/CENPX in the FA core complex. Plays a role in DNA interstrand cross-linking (ICL) repair and in recovery of replication forks stalled by topoisomerase I-DNA cleavage intermediates induced by camptothecin. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. Component of the APITD1/CENPS complex that is essential for the stable assembly of the outer kinetochore. Plays an important role in mitotic progression and chromosome segregation. Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. Belongs to the TAF9 family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; double-stranded DNA binding; chromatin binding

Biological Process: mitosis; nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; cell division; positive regulation of protein ubiquitination; resolution of meiotic joint molecules as recombinants; mitotic cell cycle; DNA repair; response to DNA damage stimulus; replication fork processing