ESPN sirna

ESPN siRNA (Human)

Cat. Num. AAA8215621

• Gene Names: ESPN; DFNB36; LP2654
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ESPN; ESPN siRNA (Human); DFNB36; Espin; Autosomal recessive deafness type 36 protein; Ectoplasmic specialization protein; ESPN sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ESPN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 854

• Applicable Applications for ESPN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ESPN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ESPN sirna

siRNA to inhibit ESPN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 110431370
• NCBI GeneID: 83715
• NCBI Accession #: NP_113663.2
• NCBI GenBank Nucleotide #: NM_031475.2
• UniProt Accession #: B1AK53; Q6XYB2; Q9H0A2; Q9Y329
• Molecular Weight: 31,636 Da
• NCBI Official Full Name: espin
• NCBI Official Synonym Full Names: espin
• NCBI Official Symbol: ESPN
• NCBI Official Synonym Symbols: DFNB36; LP2654
• NCBI Protein Information: espin
• UniProt Protein Name: Espin
• Protein Family: Espin
• UniProt Gene Name: ESPN
• UniProt Synonym Gene Names: DFNB36
• UniProt Entry Name: ESPN_HUMAN

NCBI Description

This gene encodes a multifunctional actin-bundling protein. It plays a major role in regulating the organization, dimensions, dynamics, and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in various mechanosensory and chemosensory cells. Mutations in this gene are associated with autosomal recessive neurosensory deafness, and autosomal dominant sensorineural deafness without vestibular involvement. [provided by RefSeq, Nov 2009]

Uniprot Description

ESPN: Multifunctional actin-bundling protein. Plays a major role in regulating the organization, dimensions, dynamics and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in variouS mechanosensory and chemosensory cells. Defects in ESPN are the cause of deafness autosomal recessive type 36 with or without vestibular involvement (DFNB36). A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Actin-binding

Chromosomal Location of Human Ortholog: 1p36.31

Cellular Component: filamentous actin; stereocilium; cytoplasm; stereocilium bundle tip; brush border

Molecular Function: actin filament binding; SH3 domain binding

Biological Process: positive regulation of filopodium formation; parallel actin filament bundle formation; sensory perception of sound; locomotory behavior; negative regulation of cytoskeleton organization and biogenesis

Disease: Deafness, Autosomal Recessive 36, With Or Without Vestibular Involvement