ZBTB32 sirna
ZBTB32 siRNA (Mouse)
Gene Names
Zbtb32; Rog; FAXF; FAZF; PLZP; Tzfp; 4930524C15Rik
Reactivity
Mouse
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
ZBTB32; ZBTB32 siRNA (Mouse); ROG; TZFP; Zinc finger and BTB domain-containing protein 32; Repressor of GATA; Testis zinc finger protein; ZBTB32 sirna
Host
Synthetic
Reactivity
Mouse
Specificity
ZBTB32 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
465
Applicable Applications for ZBTB32 sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ZBTB32 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for ZBTB32 sirna
siRNA to inhibit ZBTB32 expression using RNA interference
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,021 Da
NCBI Official Full Name
zinc finger and BTB domain-containing protein 32
NCBI Official Synonym Full Names
zinc finger and BTB domain containing 32
NCBI Official Symbol
Zbtb32
NCBI Official Synonym Symbols
Rog; FAXF; FAZF; PLZP; Tzfp; 4930524C15Rik
NCBI Protein Information
zinc finger and BTB domain-containing protein 32
UniProt Protein Name
Zinc finger and BTB domain-containing protein 32
Protein Family
UniProt Gene Name
Zbtb32
UniProt Synonym Gene Names
Rog; Tzfp
UniProt Entry Name
ZBT32_MOUSE
Uniprot Description
ZBTB32: DNA-binding protein that binds to the to a 5'- TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells. Belongs to the krueppel C2H2-type zinc-finger protein family.
Protein type: C2H2-type zinc finger protein; DNA-binding
Cellular Component: nuclear chromosome; nucleus
Molecular Function: DNA binding; nucleic acid binding; zinc ion binding; metal ion binding; transcription corepressor activity
Biological Process: T cell proliferation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; hemopoiesis; regulation of cytokine production
Research Articles on ZBTB32
Similar Products
Product Notes
The ZBTB32 zbtb32 (Catalog #AAA8215515) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The ZBTB32 siRNA (Mouse) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZBTB32 can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the ZBTB32 zbtb32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZBTB32, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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