RYK sirna

RYK siRNA (Human)

Cat. Num. AAA8215348

• Gene Names: RYK; JTK5; RYK1; JTK5A; D3S3195
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RYK; RYK siRNA (Human); JTK5A; Tyrosine-protein kinase RYK; RYK sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RYK siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 610

• Applicable Applications for RYK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RYK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RYK sirna

siRNA to inhibit RYK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 54607020
• NCBI GeneID: 6259
• NCBI Accession #: NP_001005861.1
• NCBI GenBank Nucleotide #: NM_001005861.2
• UniProt Accession #: P34925; Q04696
• Molecular Weight: 67,764 Da
• NCBI Official Full Name: tyrosine-protein kinase RYK isoform 1
• NCBI Official Synonym Full Names: receptor-like tyrosine kinase
• NCBI Official Symbol: RYK
• NCBI Official Synonym Symbols: JTK5; RYK1; JTK5A; D3S3195
• NCBI Protein Information: tyrosine-protein kinase RYK
• UniProt Protein Name: Tyrosine-protein kinase RYK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: RYK
• UniProt Synonym Gene Names: JTK5A
• UniProt Entry Name: RYK_HUMAN

NCBI Description

The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]

Uniprot Description

RYK: a receptor tyrosine kinase of the Ryk family. Associates with, and may modulate the signaling of, EphB2 and EphB3. Expressed in nestin-positive progenitor cells and neurons, some oligodendrocytes, O-2A progenitor cells, and type-2 astrocytes in developing CNS.

Protein type: Protein kinase, tyrosine (receptor); EC 2.7.10.1; Protein kinase, TK; Membrane protein, integral; Kinase, protein; TK group; Ryk family

Chromosomal Location of Human Ortholog: 3q22

Cellular Component: membrane; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane; nucleus

Molecular Function: protein binding; transmembrane receptor activity; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: neuron differentiation; corpus callosum development; axon guidance; peptidyl-tyrosine phosphorylation; Wnt receptor signaling pathway; positive regulation of MAPKKK cascade; signal transduction; protein amino acid phosphorylation; neurite development