FAU sirna

FAU siRNA (Mouse)

Cat. Num. AAA8215171

• Gene Names: Fau; MNSFbeta
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FAU; FAU siRNA (Mouse); Ubiquitin-like protein FUBI; Monoclonal non-specific suppressor factor beta; MNSF-beta; FAU sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FAU siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 133

• Applicable Applications for FAU sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FAU gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FAU sirna

siRNA to inhibit FAU expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 237681133
• NCBI GeneID: 14109
• NCBI Accession #: NP_001153711.1
• NCBI GenBank Nucleotide #: NM_001160239.2
• UniProt Accession #: P35545; Q9JJ24; P62862
• Molecular Weight: 7,786 Da
• NCBI Official Full Name: 40S ribosomal protein S30
• NCBI Official Synonym Full Names: Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed (fox derived)
• NCBI Official Symbol: Fau
• NCBI Official Synonym Symbols: MNSFbeta
• NCBI Protein Information: 40S ribosomal protein S30
• UniProt Protein Name: Ubiquitin-like protein FUBI
• Protein Family: 40S ribosomal protein
• UniProt Gene Name: Fau
• UniProt Synonym Gene Names: MNSF-beta
• UniProt Entry Name: UBIM_MOUSE

Uniprot Description

UBIM: This gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome. Pseudogenes derived from this gene are present in the genome. Similar to ribosomal protein S30, ribosomal proteins S27a and L40 are synthesized as fusion proteins with ubiquitin. [provided by RefSeq, Jul 2008]

Protein type: Ubiquitin-like modifier

Cellular Component: extracellular space; small ribosomal subunit

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: defense response to Gram-positive bacterium; innate immune response in mucosa; antibacterial humoral response